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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F14%3A%230001150%21RIV15-MZE-00027162
rdf:type
n9:Vysledek skos:Concept
dcterms:description
The analytical performance and evaluation of a kit-based ELISA for the determination of acrylamide in fried potato and corn chip samples are described. The sample homogenate is subjected to clean-up using SPE, followed by analyte derivatization and ELISA detection. Accuracy, precision and linearity of the ELISA procedure have been validated using spiked samples. Analytical recovery ranged from 91.8% to 96.0% with coefficients of variation below 15%. Good linearity over a wide range of dilution and minimal assay drift was observed within a microtiter plate. IC50 value of the calibration curve was 110 ng/mL, with the limit of detection about 5 ng/mL and dynamic range from 10 to 1000 ng/mL. The high specificity of the ELISA was demonstrated by cross-reactivity study using 11 potential cross-reactants. A good correlation between the results obtained from the ELISA and GC-MS within the concentration range 120-1500 mu g/kg was found in the chip samples (r=0.992, n=120). The data demonstrate that the evaluated and validated ELISA has a potential utility in a quick, simple and reliable acrylamide screening analysis for the medium-and large-sized food companies, as well as for residue laboratories and the food industry dealing with improving the chemical safety of foods available to the consumer. (c) 2014 Elsevier B.V. All rights reserved. The analytical performance and evaluation of a kit-based ELISA for the determination of acrylamide in fried potato and corn chip samples are described. The sample homogenate is subjected to clean-up using SPE, followed by analyte derivatization and ELISA detection. Accuracy, precision and linearity of the ELISA procedure have been validated using spiked samples. Analytical recovery ranged from 91.8% to 96.0% with coefficients of variation below 15%. Good linearity over a wide range of dilution and minimal assay drift was observed within a microtiter plate. IC50 value of the calibration curve was 110 ng/mL, with the limit of detection about 5 ng/mL and dynamic range from 10 to 1000 ng/mL. The high specificity of the ELISA was demonstrated by cross-reactivity study using 11 potential cross-reactants. A good correlation between the results obtained from the ELISA and GC-MS within the concentration range 120-1500 mu g/kg was found in the chip samples (r=0.992, n=120). The data demonstrate that the evaluated and validated ELISA has a potential utility in a quick, simple and reliable acrylamide screening analysis for the medium-and large-sized food companies, as well as for residue laboratories and the food industry dealing with improving the chemical safety of foods available to the consumer. (c) 2014 Elsevier B.V. All rights reserved.
dcterms:title
Analytical evaluation of a high-throughput enzyme-linked immunosorbent assay for acrylamide determination in fried foods Analytical evaluation of a high-throughput enzyme-linked immunosorbent assay for acrylamide determination in fried foods
skos:prefLabel
Analytical evaluation of a high-throughput enzyme-linked immunosorbent assay for acrylamide determination in fried foods Analytical evaluation of a high-throughput enzyme-linked immunosorbent assay for acrylamide determination in fried foods
skos:notation
RIV/00027162:_____/14:#0001150!RIV15-MZE-00027162
n3:aktivita
n17:Z n17:P
n3:aktivity
P(ED0006/01/01), Z(MZE0002716202)
n3:cisloPeriodika
JUN
n3:dodaniDat
n13:2015
n3:domaciTvurceVysledku
n12:6844758 n12:2533316
n3:druhVysledku
n18:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
2888
n3:idVysledku
RIV/00027162:_____/14:#0001150
n3:jazykVysledku
n8:eng
n3:klicovaSlova
Acrylamide; Screening method; ELISA kit; Validation; Chip samples; Food industry
n3:klicoveSlovo
n4:Food%20industry n4:ELISA%20kit n4:Chip%20samples n4:Acrylamide n4:Validation n4:Screening%20method
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[D6FB5E170AEB]
n3:nazevZdroje
Talanta
n3:obor
n19:GM
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n5:ED0006%2F01%2F01
n3:rokUplatneniVysledku
n13:2014
n3:svazekPeriodika
123
n3:tvurceVysledku
Rubio, F. Diblíková, Iva Fránek, Milan Rubio, D.
n3:wos
000335616300021
n3:zamer
n16:MZE0002716202
s:issn
0039-9140
s:numberOfPages
5
n10:doi
10.1016/j.talanta.2014.02.007