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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F14%3A%230001138%21RIV15-MZE-00027162
rdf:type
n6:Vysledek skos:Concept
dcterms:description
Routine methods for the evaluation of sperm quality are not sufficiently useful to determine the sensitivity of sperm cells to cold shock. The aim of our preliminary study was to determine whether the sperm plasma membrane integrity evaluated by modified hypoosmotic swelling test based on simple hypoosmotic swelling test (HOS test) and eosin-nigrosin staining could be helpful in predicting the degree of boar sperm survivability during semen cryopreservation. Ejaculates collected from 24 boars and 20 bulls were used in the experiment. Fresh ejaculates were evaluated by routine sperm analysis and a modified HOS test, and subsequently frozen. Sperm cryosurvivability was defined as the percentage of motile spermatozoa that survived the freezing process. A higher percentage of sperm was recovered after the thawing of semen with a higher percentage of HOS-positive and eosin-negative sperm (P < 0.01). Both indicators were found to be correlated (r = 0.707 and r = 0.705, respectively; P < 0.01). Moreover, the percentage of HOS-positive and eosin-negative sperm was similar to the percentage of viable sperm after thawing as determined by traditional eosin-nigrosin staining in boars (50.90 +/- 9.88% and 49.31 +/- 11.63%, respectively) and bulls (55.91 +/- 10.34% and 55.63 +/- 6.64%, respectively) and both indicators showed a positive correlation (r = 0.558 and r = 0.504, respectively; P < 0.05). In conclusion, based on the obtained results, we can assume that the modified HOS test can detect differences in sperm membrane resistance which allows assessment of semen quality from the perspective of its further use, e.g. cryopreservation. Routine methods for the evaluation of sperm quality are not sufficiently useful to determine the sensitivity of sperm cells to cold shock. The aim of our preliminary study was to determine whether the sperm plasma membrane integrity evaluated by modified hypoosmotic swelling test based on simple hypoosmotic swelling test (HOS test) and eosin-nigrosin staining could be helpful in predicting the degree of boar sperm survivability during semen cryopreservation. Ejaculates collected from 24 boars and 20 bulls were used in the experiment. Fresh ejaculates were evaluated by routine sperm analysis and a modified HOS test, and subsequently frozen. Sperm cryosurvivability was defined as the percentage of motile spermatozoa that survived the freezing process. A higher percentage of sperm was recovered after the thawing of semen with a higher percentage of HOS-positive and eosin-negative sperm (P < 0.01). Both indicators were found to be correlated (r = 0.707 and r = 0.705, respectively; P < 0.01). Moreover, the percentage of HOS-positive and eosin-negative sperm was similar to the percentage of viable sperm after thawing as determined by traditional eosin-nigrosin staining in boars (50.90 +/- 9.88% and 49.31 +/- 11.63%, respectively) and bulls (55.91 +/- 10.34% and 55.63 +/- 6.64%, respectively) and both indicators showed a positive correlation (r = 0.558 and r = 0.504, respectively; P < 0.05). In conclusion, based on the obtained results, we can assume that the modified HOS test can detect differences in sperm membrane resistance which allows assessment of semen quality from the perspective of its further use, e.g. cryopreservation.
dcterms:title
Modified hypoosmotic swelling test for the assessment of boar and bull sperm sensitivity to cryopreservation Modified hypoosmotic swelling test for the assessment of boar and bull sperm sensitivity to cryopreservation
skos:prefLabel
Modified hypoosmotic swelling test for the assessment of boar and bull sperm sensitivity to cryopreservation Modified hypoosmotic swelling test for the assessment of boar and bull sperm sensitivity to cryopreservation
skos:notation
RIV/00027162:_____/14:#0001138!RIV15-MZE-00027162
n3:aktivita
n4:Z n4:P
n3:aktivity
P(ED1.1.00/02.0068), P(QI111A166), Z(MZE0002716202)
n3:cisloPeriodika
4
n3:dodaniDat
n18:2015
n3:domaciTvurceVysledku
n8:7030460 n8:9741771 n8:9185682 n8:6098525
n3:druhVysledku
n10:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
30139
n3:idVysledku
RIV/00027162:_____/14:#0001138
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Ejaculate; membrane; viability; survival test; freezing; semen analysis
n3:klicoveSlovo
n12:survival%20test n12:semen%20analysis n12:freezing n12:Ejaculate n12:membrane n12:viability
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[C7DAB1628814]
n3:nazevZdroje
Acta Veterinaria Brno
n3:obor
n13:GJ
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n16:ED1.1.00%2F02.0068 n16:QI111A166
n3:rokUplatneniVysledku
n18:2014
n3:svazekPeriodika
83
n3:tvurceVysledku
Hlavicová, Jaroslava Kunetková, Monika Přinosilová, Petra Kopecká, Věra
n3:wos
000346604900006
n3:zamer
n19:MZE0002716202
s:issn
0001-7213
s:numberOfPages
7
n5:doi
10.2754/avb201483040313