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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F14%3A%230001117%21RIV15-MZE-00027162
rdf:type
skos:Concept n18:Vysledek
dcterms:description
In this study we determined the complete nucleotide sequence of multidrug-resistance plasmid p9134, and its variants p9134dT and p9134dAT which spontaneously lost either tetracycline or both tetracycline and ampicillin resistance, respectively. The plasmids were 133,802 bp, 109,512 bp and 127,291 bp in size, respectively, and their basic backbone was similar to that of Incl plasmids. Genes coding for ampicillin (bla(TEM)), chloramphenicol (catA1), streptomycin (strA, strB), tetracycline (tetA(A)) and gentamicin (aac(3)-IV) resistance were confirmed in wild-type p9134. Moreover, a gene for hygromycine resistance (hph) and a putative gene for apramycin resistance were newly determined. In p9134dAT, a continuous sequence coding for ampicillin and tetracycline resistances was lost. Genetic rearrangements in p9134dT were more complex and 2 recombination events must have occurred. During the first one, the tetracycline resistance locus was replaced with rck, srgB, srgA, orf7 and pefl originating from Salmonella virulence plasmid pSLT. During the second one, ydjA, pifA and repC genes from p9134 were replaced with repA2, PSLT025 and PSLT026 genes from pSLT. Our findings indicate that recombination event between unrelated plasmids might be quite common and may lead to the generation and selection of plasmids both transferring antibiotic resistance and increasing virulence of their host. (C) 2014 Elsevier Inc. All rights reserved. In this study we determined the complete nucleotide sequence of multidrug-resistance plasmid p9134, and its variants p9134dT and p9134dAT which spontaneously lost either tetracycline or both tetracycline and ampicillin resistance, respectively. The plasmids were 133,802 bp, 109,512 bp and 127,291 bp in size, respectively, and their basic backbone was similar to that of Incl plasmids. Genes coding for ampicillin (bla(TEM)), chloramphenicol (catA1), streptomycin (strA, strB), tetracycline (tetA(A)) and gentamicin (aac(3)-IV) resistance were confirmed in wild-type p9134. Moreover, a gene for hygromycine resistance (hph) and a putative gene for apramycin resistance were newly determined. In p9134dAT, a continuous sequence coding for ampicillin and tetracycline resistances was lost. Genetic rearrangements in p9134dT were more complex and 2 recombination events must have occurred. During the first one, the tetracycline resistance locus was replaced with rck, srgB, srgA, orf7 and pefl originating from Salmonella virulence plasmid pSLT. During the second one, ydjA, pifA and repC genes from p9134 were replaced with repA2, PSLT025 and PSLT026 genes from pSLT. Our findings indicate that recombination event between unrelated plasmids might be quite common and may lead to the generation and selection of plasmids both transferring antibiotic resistance and increasing virulence of their host. (C) 2014 Elsevier Inc. All rights reserved.
dcterms:title
Complete sequence of multidrug resistance p9134 plasmid and its variants including natural recombinant with the virulence plasmid of Salmonella serovar Typhimurium Complete sequence of multidrug resistance p9134 plasmid and its variants including natural recombinant with the virulence plasmid of Salmonella serovar Typhimurium
skos:prefLabel
Complete sequence of multidrug resistance p9134 plasmid and its variants including natural recombinant with the virulence plasmid of Salmonella serovar Typhimurium Complete sequence of multidrug resistance p9134 plasmid and its variants including natural recombinant with the virulence plasmid of Salmonella serovar Typhimurium
skos:notation
RIV/00027162:_____/14:#0001117!RIV15-MZE-00027162
n4:aktivita
n12:P n12:Z
n4:aktivity
P(ED0006/01/01), P(GAP502/12/1467), Z(MZE0002716202)
n4:cisloPeriodika
NOV
n4:dodaniDat
n11:2015
n4:domaciTvurceVysledku
n13:7318537 n13:1703552 n13:8610266 n13:9231625
n4:druhVysledku
n8:J
n4:duvernostUdaju
n15:S
n4:entitaPredkladatele
n10:predkladatel
n4:idSjednocenehoVysledku
8345
n4:idVysledku
RIV/00027162:_____/14:#0001117
n4:jazykVysledku
n5:eng
n4:klicovaSlova
Plasmid sequence; Antibiotic resistance; Recombination; Salmonella; Conjugation
n4:klicoveSlovo
n6:Plasmid%20sequence n6:Conjugation n6:Antibiotic%20resistance n6:Recombination n6:Salmonella
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[527107EF3E87]
n4:nazevZdroje
Plasmid
n4:obor
n17:EB
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
4
n4:projekt
n9:GAP502%2F12%2F1467 n9:ED0006%2F01%2F01
n4:rokUplatneniVysledku
n11:2014
n4:svazekPeriodika
76
n4:tvurceVysledku
Kubasová, Tereza Matiašovicová, Jitka Rychlík, Ivan Juřicová, Helena
n4:wos
000345609000002
n4:zamer
n19:MZE0002716202
s:issn
0147-619X
s:numberOfPages
7
n3:doi
10.1016/j.plasmid.2014.08.003