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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F14%3A%230001103%21RIV15-MZE-00027162
rdf:type
n13:Vysledek skos:Concept
dcterms:description
The influence of specific and non-specific antibiotic pressure on in vivo spread of macrolide-lincosamide-streptogramin B (MLSB) resistance was evaluated in this study. Chickens repeatedly inoculated with Enterococcus faecalis harbouring the plasmid pAM beta 1 carrying the erm(B) gene were perorally treated for one week with tylosin, lincomycin (both specific antibiotic pressure) and chlortetracycline (non-specific antibiotic pressure). Antibiotic non-treated but E. faecalis inoculated chickens served as a control. To quantify the erm(B) gene and characterise intestinal microflora, faecal DNA was analysed by qPCR and 454-pyrosequencing. Under the pressure of antibiotics, a significant increase in erm(B) was observed by qPCR. However, at the final stage of the experiment, an increase in erm(B) was also observed in two out of five non-treated chickens. In chickens treated with tylosin and chlortetracycline, the increase in erm(B) was accompanied by an increase in enterococci. However, E. faecalis was at the limit of detection in all animals. This suggests that the erm(B) gene spread among the gut microbiota other than E. faecalis. Pyrosequencing results indicated that, depending on the particular antibiotic pressure, different bacteria could be responsible for the spread of MLSB resistance. Different species of MLSB-resistant enterococci and streptococci were isolated from cloacal swabs during and after the treatment. PFGE analysis of MLSB-resistant enterococci revealed four clones, all differing from the challenge strain. All of the MLSB-resistant isolates harboured a plasmid of the same size as pAM beta 1. This study has shown that MLSB resistance may spread within the gut microbiota under specific and non-specific pressure and even in the absence of any antimicrobial pressure. Finally, depending on the particular antibiotic pressure, different bacterial species seems to be involved in the spread of MLSB resistance. The influence of specific and non-specific antibiotic pressure on in vivo spread of macrolide-lincosamide-streptogramin B (MLSB) resistance was evaluated in this study. Chickens repeatedly inoculated with Enterococcus faecalis harbouring the plasmid pAM beta 1 carrying the erm(B) gene were perorally treated for one week with tylosin, lincomycin (both specific antibiotic pressure) and chlortetracycline (non-specific antibiotic pressure). Antibiotic non-treated but E. faecalis inoculated chickens served as a control. To quantify the erm(B) gene and characterise intestinal microflora, faecal DNA was analysed by qPCR and 454-pyrosequencing. Under the pressure of antibiotics, a significant increase in erm(B) was observed by qPCR. However, at the final stage of the experiment, an increase in erm(B) was also observed in two out of five non-treated chickens. In chickens treated with tylosin and chlortetracycline, the increase in erm(B) was accompanied by an increase in enterococci. However, E. faecalis was at the limit of detection in all animals. This suggests that the erm(B) gene spread among the gut microbiota other than E. faecalis. Pyrosequencing results indicated that, depending on the particular antibiotic pressure, different bacteria could be responsible for the spread of MLSB resistance. Different species of MLSB-resistant enterococci and streptococci were isolated from cloacal swabs during and after the treatment. PFGE analysis of MLSB-resistant enterococci revealed four clones, all differing from the challenge strain. All of the MLSB-resistant isolates harboured a plasmid of the same size as pAM beta 1. This study has shown that MLSB resistance may spread within the gut microbiota under specific and non-specific pressure and even in the absence of any antimicrobial pressure. Finally, depending on the particular antibiotic pressure, different bacterial species seems to be involved in the spread of MLSB resistance.
dcterms:title
In vivo spread of macrolide-lincosamide-streptogramin B (MLSB) resistance-A model study in chickens In vivo spread of macrolide-lincosamide-streptogramin B (MLSB) resistance-A model study in chickens
skos:prefLabel
In vivo spread of macrolide-lincosamide-streptogramin B (MLSB) resistance-A model study in chickens In vivo spread of macrolide-lincosamide-streptogramin B (MLSB) resistance-A model study in chickens
skos:notation
RIV/00027162:_____/14:#0001103!RIV15-MZE-00027162
n3:aktivita
n10:P n10:Z
n3:aktivity
P(ED0006/01/01), P(QH91231), Z(MZE0002716202)
n3:cisloPeriodika
3-4
n3:dodaniDat
n17:2015
n3:domaciTvurceVysledku
n7:1317121 n7:6666329 Maroševič, Durdica n7:2380145 n7:5144523 n7:2050986
n3:druhVysledku
n4:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
21092
n3:idVysledku
RIV/00027162:_____/14:#0001103
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Metagenomics; Susceptibility; Intestine; Microflora; 454
n3:klicoveSlovo
n6:Microflora n6:Susceptibility n6:Intestine n6:454 n6:Metagenomics
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[51BAD7860D03]
n3:nazevZdroje
Veterinary Microbiology
n3:obor
n9:EE
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n11:QH91231 n11:ED0006%2F01%2F01
n3:rokUplatneniVysledku
n17:2014
n3:svazekPeriodika
171
n3:tvurceVysledku
Červinková, Dana Vídeňská, Petra Jaglič, Zoran Maroševič, Durdica Babák, Vladimír Vlková, Hana
n3:wos
000338391700018
n3:zamer
n14:MZE0002716202
s:issn
0378-1135
s:numberOfPages
9
n15:doi
10.1016/j.vetmic.2013.12.017