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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F13%3A%230001014%21RIV14-MZE-00027162
rdf:type
n8:Vysledek skos:Concept
rdfs:seeAlso
http://www.veterinaryresearch.org/content/44/1/98
dcterms:description
Monocytes play an essential role in the defense against bacterial pathogens. Bone marrow ( BM) and peripheral blood ( PB) monocytes in pigs consist of the main %22steady-state%22 subpopulations: CD14(hi)/CD163(-)/SLA-DR- and CD14(low)/CD163(+)/SLA-DR+. During inflammation, the subpopulation of %22inflammatory%22 monocytes expressing very high levels of CD163, but lacking the SLA-DR molecule (being CD14(low)/CD163(+)/SLA-DR-) appears in the BM and PB and replaces the CD14(low)/CD163(+)/SLA-DR+ subpopulation. However, current knowledge of monocyte migration into inflamed tissues in pigs is limited. The aim of the present study was to evaluate the distribution of %22inflammatory%22 CD14(low)/CD163(+)/SLA-DR- monocytes during experimental inflammation induced by Actinobacillus pleuropneumoniae (APP) and a possible role for chemokines in attracting %22inflammatory%22 CD14(low)/CD163(+)/SLA-DR- monocytes into the tissues. Monocyte subpopulations were detected by flow cytometry. Chemokines and chemokine receptors were detected by RT-qPCR. The %22steady-state%22 monocytes were found in the BM, PB, spleen and lungs of control pigs. After APP-infection, %22inflammatory%22 monocytes replaced the %22steady-state%22 subpopulation in BM, PB, spleen and moreover, they appeared in an unaffected area, demarcation zone and necrotic area of the lungs and in tracheobronchial lymph nodes. They did not appear in mesenteric lymph nodes. Levels of mRNA for various chemokines with their appropriate receptors were found to be elevated in BM (CCL3-CCR1/CCR5, CCL8-CCR2/CCR5, CCL19-CCR7), necrotic area of the lungs (CCL3-CCR1, CCL5-CCR1/CCR3, CCL11-CCR3, CCL22/CCR4) and tracheobronchial lymph nodes (CCL3-CCR1) and therefore they could play a role in attracting monocytes into inflamed tissues. In conclusion, %22inflammatory%22 monocytes appear in different lymphoid tissues and the lungs after APP infection in pigs. Various chemokines could drive this process. Monocytes play an essential role in the defense against bacterial pathogens. Bone marrow ( BM) and peripheral blood ( PB) monocytes in pigs consist of the main %22steady-state%22 subpopulations: CD14(hi)/CD163(-)/SLA-DR- and CD14(low)/CD163(+)/SLA-DR+. During inflammation, the subpopulation of %22inflammatory%22 monocytes expressing very high levels of CD163, but lacking the SLA-DR molecule (being CD14(low)/CD163(+)/SLA-DR-) appears in the BM and PB and replaces the CD14(low)/CD163(+)/SLA-DR+ subpopulation. However, current knowledge of monocyte migration into inflamed tissues in pigs is limited. The aim of the present study was to evaluate the distribution of %22inflammatory%22 CD14(low)/CD163(+)/SLA-DR- monocytes during experimental inflammation induced by Actinobacillus pleuropneumoniae (APP) and a possible role for chemokines in attracting %22inflammatory%22 CD14(low)/CD163(+)/SLA-DR- monocytes into the tissues. Monocyte subpopulations were detected by flow cytometry. Chemokines and chemokine receptors were detected by RT-qPCR. The %22steady-state%22 monocytes were found in the BM, PB, spleen and lungs of control pigs. After APP-infection, %22inflammatory%22 monocytes replaced the %22steady-state%22 subpopulation in BM, PB, spleen and moreover, they appeared in an unaffected area, demarcation zone and necrotic area of the lungs and in tracheobronchial lymph nodes. They did not appear in mesenteric lymph nodes. Levels of mRNA for various chemokines with their appropriate receptors were found to be elevated in BM (CCL3-CCR1/CCR5, CCL8-CCR2/CCR5, CCL19-CCR7), necrotic area of the lungs (CCL3-CCR1, CCL5-CCR1/CCR3, CCL11-CCR3, CCL22/CCR4) and tracheobronchial lymph nodes (CCL3-CCR1) and therefore they could play a role in attracting monocytes into inflamed tissues. In conclusion, %22inflammatory%22 monocytes appear in different lymphoid tissues and the lungs after APP infection in pigs. Various chemokines could drive this process.
dcterms:title
Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines
skos:prefLabel
Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines
skos:notation
RIV/00027162:_____/13:#0001014!RIV14-MZE-00027162
n8:predkladatel
n14:ico%3A00027162
n3:aktivita
n12:Z n12:P
n3:aktivity
P(ED0006/01/01), P(GPP502/10/P362), Z(MZE0002716202)
n3:cisloPeriodika
98
n3:dodaniDat
n15:2014
n3:domaciTvurceVysledku
n4:1862758 n4:5520126 n4:6502415 n4:7153643 n4:9627650 n4:5548721
n3:druhVysledku
n17:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
70000
n3:idVysledku
RIV/00027162:_____/13:#0001014
n3:jazykVysledku
n21:eng
n3:klicovaSlova
BONE-MARROW; SPLENIC RESERVOIR; DENDRITIC CELLS; T-CELLS; EXPRESSION; NODES; SUBPOPULATIONS; INFLAMMATION; SALMONELLA; EMIGRATION
n3:klicoveSlovo
n5:EXPRESSION n5:INFLAMMATION n5:BONE-MARROW n5:SALMONELLA n5:EMIGRATION n5:NODES n5:SPLENIC%20RESERVOIR n5:SUBPOPULATIONS n5:T-CELLS n5:DENDRITIC%20CELLS
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[CC654368004F]
n3:nazevZdroje
Veterinary Research
n3:obor
n10:EC
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n9:ED0006%2F01%2F01 n9:GPP502%2F10%2FP362
n3:rokUplatneniVysledku
n15:2013
n3:svazekPeriodika
44
n3:tvurceVysledku
Faldyna, Martin Ondráčková, Petra Levá, Lenka Menšíková, Markéta Kučerová, Zdeňka Vícenová, Monika
n3:wos
000326186800001
n3:zamer
n18:MZE0002716202
s:issn
1297-9716
s:numberOfPages
16
n16:doi
10.1186/1297-9716-44-98