This HTML5 document contains 52 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n8http://linked.opendata.cz/resource/domain/vavai/projekt/
n7http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n14http://linked.opendata.cz/resource/domain/vavai/subjekt/
n12http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
skoshttp://www.w3.org/2004/02/skos/core#
n10http://bibframe.org/vocab/
n17http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00027162%3A_____%2F13%3A%230000964%21RIV14-MZE-00027162/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n6http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n13http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n16http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n11http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n5http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F13%3A%230000964%21RIV14-MZE-00027162
rdf:type
skos:Concept n12:Vysledek
dcterms:description
The virulence factors EAST1 and AIDA are often detected in ETEC/VTEC strains isolated from pigs and their role in diarrhoeal infections is discussed. In order to elucidate the pathogenesis of AIDA, the colonisation patterns of F4 positive and AIDA positive strains were investigated. Two wild-type Escherichia coli strains AIDA/EAST1 and F4/EAST1 isolated from diarrhoeal piglets were used for animal experiment to evaluate the ability of the EAST1 toxin to be involved in induction of diarrhoea. Gnotobiotic piglets were supplemented with normal porcine serum and orally inoculated with the strains. Faecal bacterial shedding of the challenge strains was observed during the experiment. Light microscopy and scanning electron microscopy were used to detect the colonisation pattern of both challenge strains. Although bacterial isolation demonstrated shedding of the challenge strains until the end of the experiment, diarrhoea did not develop in any piglet. Based on histological examination, piglets were more heavily colonised in the case of infection with E. coli O149/F4/EAST1 strain. Scanning electron microscopy showed bacterial cells of F4/EAST1 E. coli adhering to enterocytes, in contrast to AIDA/EAST1 which were poorly present on the intestinal surface. The EAST1 toxin alone was not able to induce diarrhoea in animals. Therefore our results demonstrate that the function/role of EAST1 and AIDA in colibacillosis of pigs remains to be elucidated. The virulence factors EAST1 and AIDA are often detected in ETEC/VTEC strains isolated from pigs and their role in diarrhoeal infections is discussed. In order to elucidate the pathogenesis of AIDA, the colonisation patterns of F4 positive and AIDA positive strains were investigated. Two wild-type Escherichia coli strains AIDA/EAST1 and F4/EAST1 isolated from diarrhoeal piglets were used for animal experiment to evaluate the ability of the EAST1 toxin to be involved in induction of diarrhoea. Gnotobiotic piglets were supplemented with normal porcine serum and orally inoculated with the strains. Faecal bacterial shedding of the challenge strains was observed during the experiment. Light microscopy and scanning electron microscopy were used to detect the colonisation pattern of both challenge strains. Although bacterial isolation demonstrated shedding of the challenge strains until the end of the experiment, diarrhoea did not develop in any piglet. Based on histological examination, piglets were more heavily colonised in the case of infection with E. coli O149/F4/EAST1 strain. Scanning electron microscopy showed bacterial cells of F4/EAST1 E. coli adhering to enterocytes, in contrast to AIDA/EAST1 which were poorly present on the intestinal surface. The EAST1 toxin alone was not able to induce diarrhoea in animals. Therefore our results demonstrate that the function/role of EAST1 and AIDA in colibacillosis of pigs remains to be elucidated.
dcterms:title
Experimental infection of gnotobiotic piglets with Escherichia coli strains positive for EAST1 and AIDA Experimental infection of gnotobiotic piglets with Escherichia coli strains positive for EAST1 and AIDA
skos:prefLabel
Experimental infection of gnotobiotic piglets with Escherichia coli strains positive for EAST1 and AIDA Experimental infection of gnotobiotic piglets with Escherichia coli strains positive for EAST1 and AIDA
skos:notation
RIV/00027162:_____/13:#0000964!RIV14-MZE-00027162
n12:predkladatel
n14:ico%3A00027162
n4:aktivita
n9:P
n4:aktivity
P(ED0006/01/01), P(QH71055)
n4:cisloPeriodika
1-2
n4:dodaniDat
n5:2014
n4:domaciTvurceVysledku
n7:7988370 n7:6757669 n7:3923673 n7:2833174 n7:4214064 n7:7153643
n4:druhVysledku
n11:J
n4:duvernostUdaju
n18:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
74048
n4:idVysledku
RIV/00027162:_____/13:#0000964
n4:jazykVysledku
n13:eng
n4:klicovaSlova
Escherichia coli; Virulence factors; EAST1; AIDA; Experimental infection
n4:klicoveSlovo
n6:AIDA n6:Experimental%20infection n6:Escherichia%20coli n6:Virulence%20factors n6:EAST1
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[F10FB618A7D5]
n4:nazevZdroje
Veterinary Immunology and Immunopathology
n4:obor
n16:EE
n4:pocetDomacichTvurcuVysledku
6
n4:pocetTvurcuVysledku
6
n4:projekt
n8:ED0006%2F01%2F01 n8:QH71055
n4:rokUplatneniVysledku
n5:2013
n4:svazekPeriodika
152
n4:tvurceVysledku
Kulich, Pavel Šrámková-Zajacová, Zuzana Mašková, Jarmila Faldyna, Martin Alexa, Pavel Kummer, Vladimír
n4:wos
000316535000024
s:issn
0165-2427
s:numberOfPages
6
n10:doi
10.1016/j.vetimm.2012.09.011