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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F12%3A%230000922%21RIV13-MZE-00027162
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Very little is known about the occurrence of immune system cells in the canine uterus. The aim of this study was to generate information about lymphocyte subsets that are present in the healthy canine uterus and that are recruited under inflammatory conditions caused by pyometra. Using immunohistochemistry and flow cytometry, a significant influx of ?? T lymphocytes was found in pyometra samples mainly due to recruitment of ??(+)/CD8(-) T lymphocytes. The relative expression of genes encoding selected cytokines/chemokines was evaluated in samples from healthy and pyometra-affected uteri. Expression of pro-inflammatory cytokines (including IL-1ß, TNF-?, IL-8, IL-17 and IFN-?) and chemokines (including CXCL10, CCL4 and CCL5) was upregulated in pyometra samples confirming the presence of inflammation. In contrast, the expression of the homeostatic chemokine CCL25 and of the anti-inflammatory cytokine IL-10 was downregulated and unchanged, respectively. Copyright c 2012 Elsevier Ltd. All rights reserved. Very little is known about the occurrence of immune system cells in the canine uterus. The aim of this study was to generate information about lymphocyte subsets that are present in the healthy canine uterus and that are recruited under inflammatory conditions caused by pyometra. Using immunohistochemistry and flow cytometry, a significant influx of ?? T lymphocytes was found in pyometra samples mainly due to recruitment of ??(+)/CD8(-) T lymphocytes. The relative expression of genes encoding selected cytokines/chemokines was evaluated in samples from healthy and pyometra-affected uteri. Expression of pro-inflammatory cytokines (including IL-1ß, TNF-?, IL-8, IL-17 and IFN-?) and chemokines (including CXCL10, CCL4 and CCL5) was upregulated in pyometra samples confirming the presence of inflammation. In contrast, the expression of the homeostatic chemokine CCL25 and of the anti-inflammatory cytokine IL-10 was downregulated and unchanged, respectively. Copyright c 2012 Elsevier Ltd. All rights reserved.
dcterms:title
Gamma delta T lymphocytes are recruited into the inflamed uterus of bitches suffering from pyometra Gamma delta T lymphocytes are recruited into the inflamed uterus of bitches suffering from pyometra
skos:prefLabel
Gamma delta T lymphocytes are recruited into the inflamed uterus of bitches suffering from pyometra Gamma delta T lymphocytes are recruited into the inflamed uterus of bitches suffering from pyometra
skos:notation
RIV/00027162:_____/12:#0000922!RIV13-MZE-00027162
n8:predkladatel
n9:ico%3A00027162
n3:aktivita
n11:P n11:Z
n3:aktivity
P(ED0006/01/01), Z(MZE0002716202)
n3:cisloPeriodika
3
n3:dodaniDat
n15:2013
n3:domaciTvurceVysledku
Oreskovič, Zrinka n7:6502415 n7:7331614 n7:7362366 n7:5548721 n7:7153643 n7:9627650 n7:7911939
n3:druhVysledku
n6:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
137703
n3:idVysledku
RIV/00027162:_____/12:#0000922
n3:jazykVysledku
n20:eng
n3:klicovaSlova
Canine; Lymphocyte subsets; Flow cytometry; Chemokines; gamma delta Lymphocytes
n3:klicoveSlovo
n4:gamma%20delta%20Lymphocytes n4:Flow%20cytometry n4:Canine n4:Chemokines n4:Lymphocyte%20subsets
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[869E54FE7CE9]
n3:nazevZdroje
Veterinary Journal
n3:obor
n5:GJ
n3:pocetDomacichTvurcuVysledku
8
n3:pocetTvurcuVysledku
11
n3:projekt
n10:ED0006%2F01%2F01
n3:rokUplatneniVysledku
n15:2012
n3:svazekPeriodika
194
n3:tvurceVysledku
Matiašovic, Ján Bartošková, A. Štěpánová, Hana Levá, Lenka Vícenová, Monika Vitásek, R. Faldyna, Martin Oreskovič, Zrinka Turánek-Knötigová, Pavlína Moore, P. F. Ondráčková, Petra
n3:wos
000313224300012
n3:zamer
n19:MZE0002716202
s:issn
1090-0233
s:numberOfPages
6
n18:doi
10.1016/j.tvjl.2012.05.024