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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F12%3A%230000917%21RIV13-MZE-00027162
rdf:type
n4:Vysledek skos:Concept
dcterms:description
In the veterinary field, only limited information is available about interleukin-17A (IL-17), despite the fact that this cytokine plays an important role during pro-inflammatory immune responses and induces the production of chemotactic factors for neutrophils. The aim of this study was to characterize porcine IL-17-producing cells. We tested the cross-reactivity of five anti-human IL-17 monoclonal antibodies because such antibodies against porcine IL-17 are currently unavailable. Whole blood cells (WBCs) were stimulated with phorbol-myristate-acetate (PMA) and ionomycin and subsequently analyzed by flow cytometry. The antibody clone SCPL1362 was found to cross-react with porcine IL-17, whereas the other four antibodies tested did not recognize this cytokine. Using this antibody, we characterized porcine WBC-secreting IL-17 after PMA and ionomycin stimulation. All IL-17-producing WBCs were positive for the T lymphocyte marker CD3. Myeloid cells (CD172 alpha(+)) and B lymphocytes (CD79 alpha(+)) were IL-17 negative. The major subset of IL-17 positive T lymphocytes was the CD4(+) lymphocytes (about 60% of all IL-17 positive WBCs). The remaining IL-17 positive WBCs were gamma delta TCR+ lymphocytes. CD8 positive and CD8 negative cells were found within both CD4(+) and gamma delta TCR+ cells producing the cytokine. Moreover, IL-17 positive cells were mostly CD45RA negative, therefore activated cells or memory cells. Flow cytometry data were confirmed using sorted cells. Both sorted CD4(+) and gamma delta TCR+ cells produced IL-17 at mRNA level after PMA and ionomycin stimulation while double negative CD4(-)gamma delta TCR- cells were negative for IL-17. We can conclude that only two subpopulations of porcine WBCs are sources of IL-17 after non-specific stimulation: CD3(+)CD4(+) and CD3(+)gamma delta TCR+. (C) 2012. In the veterinary field, only limited information is available about interleukin-17A (IL-17), despite the fact that this cytokine plays an important role during pro-inflammatory immune responses and induces the production of chemotactic factors for neutrophils. The aim of this study was to characterize porcine IL-17-producing cells. We tested the cross-reactivity of five anti-human IL-17 monoclonal antibodies because such antibodies against porcine IL-17 are currently unavailable. Whole blood cells (WBCs) were stimulated with phorbol-myristate-acetate (PMA) and ionomycin and subsequently analyzed by flow cytometry. The antibody clone SCPL1362 was found to cross-react with porcine IL-17, whereas the other four antibodies tested did not recognize this cytokine. Using this antibody, we characterized porcine WBC-secreting IL-17 after PMA and ionomycin stimulation. All IL-17-producing WBCs were positive for the T lymphocyte marker CD3. Myeloid cells (CD172 alpha(+)) and B lymphocytes (CD79 alpha(+)) were IL-17 negative. The major subset of IL-17 positive T lymphocytes was the CD4(+) lymphocytes (about 60% of all IL-17 positive WBCs). The remaining IL-17 positive WBCs were gamma delta TCR+ lymphocytes. CD8 positive and CD8 negative cells were found within both CD4(+) and gamma delta TCR+ cells producing the cytokine. Moreover, IL-17 positive cells were mostly CD45RA negative, therefore activated cells or memory cells. Flow cytometry data were confirmed using sorted cells. Both sorted CD4(+) and gamma delta TCR+ cells produced IL-17 at mRNA level after PMA and ionomycin stimulation while double negative CD4(-)gamma delta TCR- cells were negative for IL-17. We can conclude that only two subpopulations of porcine WBCs are sources of IL-17 after non-specific stimulation: CD3(+)CD4(+) and CD3(+)gamma delta TCR+. (C) 2012.
dcterms:title
CD4(+) and gamma delta TCR+ T lymphocytes are sources of interleukin-17 in swine CD4(+) and gamma delta TCR+ T lymphocytes are sources of interleukin-17 in swine
skos:prefLabel
CD4(+) and gamma delta TCR+ T lymphocytes are sources of interleukin-17 in swine CD4(+) and gamma delta TCR+ T lymphocytes are sources of interleukin-17 in swine
skos:notation
RIV/00027162:_____/12:#0000917!RIV13-MZE-00027162
n4:predkladatel
n20:ico%3A00027162
n3:aktivita
n14:P n14:Z
n3:aktivity
P(ED0006/01/01), P(GA524/08/1606), Z(MZE0002716202)
n3:cisloPeriodika
2
n3:dodaniDat
n5:2013
n3:domaciTvurceVysledku
n7:3079562 n7:7331614 n7:7153643 n7:1862758
n3:druhVysledku
n16:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
126170
n3:idVysledku
RIV/00027162:_____/12:#0000917
n3:jazykVysledku
n6:eng
n3:klicovaSlova
ACTINOBACILLUS-PLEUROPNEUMONIAE INFECTION; SALMONELLA-ENTERICA; LYMPHOID-TISSUE; IMMUNE-SYSTEM; CELLS; PIGLETS; EXPRESSION; IL-17; PIGS; DISTINCT
n3:klicoveSlovo
n8:ACTINOBACILLUS-PLEUROPNEUMONIAE%20INFECTION n8:PIGS n8:CELLS n8:SALMONELLA-ENTERICA n8:PIGLETS n8:EXPRESSION n8:DISTINCT n8:IL-17 n8:IMMUNE-SYSTEM n8:LYMPHOID-TISSUE
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[7467CA73D848]
n3:nazevZdroje
Cytokine
n3:obor
n13:GJ
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n11:GA524%2F08%2F1606 n11:ED0006%2F01%2F01
n3:rokUplatneniVysledku
n5:2012
n3:svazekPeriodika
58
n3:tvurceVysledku
Menšíková, Markéta Chlebová, Katarína Štěpánová, Hana Faldyna, Martin
n3:wos
000303099200003
n3:zamer
n17:MZE0002716202
s:issn
1043-4666
s:numberOfPages
6
n10:doi
10.1016/j.cyto.2012.01.004