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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F12%3A%230000916%21RIV13-MZE-00027162
rdf:type
n3:Vysledek skos:Concept
dcterms:description
Adenosine is a well described anti-inflammatory modulator of immune responses. The aim of the present study was to describe the role of common adenosine agonist 5'-N-ethylcarboxamidoadenosine (NECA) in cytokine production by main porcine T cell subpopulations. TNF-alpha, IFN-gamma, IL-2 and IL-10 were detected by multicolor flow cytometry together with cell surface markers CD3, CD4 and CD8. It was found that NECA inhibits (in a dose-dependent manner) production of pro-inflammatory TNF-alpha and Th1-associated cytokines IFN-gamma, IL-2 in all concanavalin A-stimulated T cell subpopulations. Moreover, production of IL-10 was potentiated in all T cell subpopulations tested. These corresponded well with the fact that all T cell subsets expressed mRNA for adenosine receptor (AR) subtypes to comparable extents. Contrary to concanavalin A-stimulated cells, NECA had a moderate effect on PMA-stimulated T cells, suggesting that AR in pigs acts via signaling pathways not associated with protein-kinase C. Non-selective antagonist CGS15943 as well as allosteric modulator SCH202676 failed to reverse the effect of NECA in pigs. In conclusion, NECA has an anti-inflammatory effect on porcine T cell subpopulations. Adenosine is a well described anti-inflammatory modulator of immune responses. The aim of the present study was to describe the role of common adenosine agonist 5'-N-ethylcarboxamidoadenosine (NECA) in cytokine production by main porcine T cell subpopulations. TNF-alpha, IFN-gamma, IL-2 and IL-10 were detected by multicolor flow cytometry together with cell surface markers CD3, CD4 and CD8. It was found that NECA inhibits (in a dose-dependent manner) production of pro-inflammatory TNF-alpha and Th1-associated cytokines IFN-gamma, IL-2 in all concanavalin A-stimulated T cell subpopulations. Moreover, production of IL-10 was potentiated in all T cell subpopulations tested. These corresponded well with the fact that all T cell subsets expressed mRNA for adenosine receptor (AR) subtypes to comparable extents. Contrary to concanavalin A-stimulated cells, NECA had a moderate effect on PMA-stimulated T cells, suggesting that AR in pigs acts via signaling pathways not associated with protein-kinase C. Non-selective antagonist CGS15943 as well as allosteric modulator SCH202676 failed to reverse the effect of NECA in pigs. In conclusion, NECA has an anti-inflammatory effect on porcine T cell subpopulations.
dcterms:title
The effect of adenosine on pro-inflammatory cytokine production by porcine T cells The effect of adenosine on pro-inflammatory cytokine production by porcine T cells
skos:prefLabel
The effect of adenosine on pro-inflammatory cytokine production by porcine T cells The effect of adenosine on pro-inflammatory cytokine production by porcine T cells
skos:notation
RIV/00027162:_____/12:#0000916!RIV13-MZE-00027162
n3:predkladatel
n6:ico%3A00027162
n4:aktivita
n20:P n20:Z
n4:aktivity
P(ED0006/01/01), P(IAA601680801), Z(MSM0021620849), Z(MZE0002716202)
n4:cisloPeriodika
1-2
n4:dodaniDat
n8:2013
n4:domaciTvurceVysledku
n13:7362366 n13:7153643 n13:6502415 n13:5548721
n4:druhVysledku
n19:J
n4:duvernostUdaju
n10:S
n4:entitaPredkladatele
n9:predkladatel
n4:idSjednocenehoVysledku
132914
n4:idVysledku
RIV/00027162:_____/12:#0000916
n4:jazykVysledku
n17:eng
n4:klicovaSlova
Pig; T cell subpopulations; Cytokines; 5 '-N-ethylcarboxamidoadenosine; Adenosine receptor subtypes
n4:klicoveSlovo
n11:T%20cell%20subpopulations n11:Pig n11:5%20%27-N-ethylcarboxamidoadenosine n11:Cytokines n11:Adenosine%20receptor%20subtypes
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[6EEEF0DA6F06]
n4:nazevZdroje
Veterinary Immunology and Immunopathology
n4:obor
n5:EC
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
6
n4:projekt
n14:IAA601680801 n14:ED0006%2F01%2F01
n4:rokUplatneniVysledku
n8:2012
n4:svazekPeriodika
145
n4:tvurceVysledku
Faldyna, Martin Ondráčková, Petra Kovářů, F. Levá, Lenka Kovářů, H. Matiašovic, Ján
n4:wos
000301159200038
n4:zamer
n18:MZE0002716202 n18:MSM0021620849
s:issn
0165-2427
s:numberOfPages
8
n15:doi
10.1016/j.vetimm.2011.12.002