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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F12%3A%230000915%21RIV13-MZE-00027162
rdf:type
n7:Vysledek skos:Concept
dcterms:description
Using two-color flow cytometry, we characterized CD34(+) cells in the newborn canine thymus. CD34(+) thymic cells comprised approximately 5% of cells recovered by thymus tissue teasing and both large and small thymocytes have been present in this population, the former being 7-12 times more frequent. All CD34(+) cells expressed the pan-leukocyte antigen CD45. The expression of CD44 profile on the large and small CD34(+) thymocytes differed: almost all large CD34(+) cells were CD44(+), while only 75% of small CD34(+) thymocytes co-expressed the CD44 antigen. We have previously described that CD172 alpha is present on the surface of CD34(+) bone marrow cells in dogs. In the thymus, CD172 alpha was expressed on 5-10% and less than 5% of large and small CD34(+) cells, respectively. Some CD34(+) thymocytes also co-expressed T-lineage-specific markers like CD3, CD4, CD8,TCR1 and TCR2. Their expression increased during the large-to-small thymocyte transition. Based on our findings we suggest that thymocyte progenitors enter their primary differentiation center as large CD34(+), CD44(+), CD45(+) and CD172 alpha(+) cells. T-cell specific markers appear on their surface at early stages of differentiation. As the size of progenitors decreases with terminal primary differentiation, the CD34. CD44, and CD172 alpha surface markers are down-regulated. Using two-color flow cytometry, we characterized CD34(+) cells in the newborn canine thymus. CD34(+) thymic cells comprised approximately 5% of cells recovered by thymus tissue teasing and both large and small thymocytes have been present in this population, the former being 7-12 times more frequent. All CD34(+) cells expressed the pan-leukocyte antigen CD45. The expression of CD44 profile on the large and small CD34(+) thymocytes differed: almost all large CD34(+) cells were CD44(+), while only 75% of small CD34(+) thymocytes co-expressed the CD44 antigen. We have previously described that CD172 alpha is present on the surface of CD34(+) bone marrow cells in dogs. In the thymus, CD172 alpha was expressed on 5-10% and less than 5% of large and small CD34(+) cells, respectively. Some CD34(+) thymocytes also co-expressed T-lineage-specific markers like CD3, CD4, CD8,TCR1 and TCR2. Their expression increased during the large-to-small thymocyte transition. Based on our findings we suggest that thymocyte progenitors enter their primary differentiation center as large CD34(+), CD44(+), CD45(+) and CD172 alpha(+) cells. T-cell specific markers appear on their surface at early stages of differentiation. As the size of progenitors decreases with terminal primary differentiation, the CD34. CD44, and CD172 alpha surface markers are down-regulated.
dcterms:title
Characterization of CD34(+) thymocytes in newborn dogs Characterization of CD34(+) thymocytes in newborn dogs
skos:prefLabel
Characterization of CD34(+) thymocytes in newborn dogs Characterization of CD34(+) thymocytes in newborn dogs
skos:notation
RIV/00027162:_____/12:#0000915!RIV13-MZE-00027162
n7:predkladatel
n8:ico%3A00027162
n3:aktivita
n17:V n17:Z n17:P
n3:aktivity
P(ED0006/01/01), V, Z(MZE0002716202)
n3:cisloPeriodika
1-2
n3:dodaniDat
n5:2013
n3:domaciTvurceVysledku
n10:6502415 n10:8138532 n10:7153643
n3:druhVysledku
n19:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
126805
n3:idVysledku
RIV/00027162:_____/12:#0000915
n3:jazykVysledku
n4:eng
n3:klicovaSlova
Canine; Thymopoiesis; Flow cytometry; CD34(+)
n3:klicoveSlovo
n18:Flow%20cytometry n18:Canine n18:CD34%28%2B%29 n18:Thymopoiesis
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[464FB15AF428]
n3:nazevZdroje
Veterinary Immunology and Immunopathology
n3:obor
n9:GJ
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
5
n3:projekt
n12:ED0006%2F01%2F01
n3:rokUplatneniVysledku
n5:2012
n3:svazekPeriodika
147
n3:tvurceVysledku
Šinkora, J. Toman, Miroslav Šinkorová, Z. Faldyna, Martin Levá, Lenka
n3:wos
000304687600010
n3:zamer
n15:MZE0002716202
s:issn
0165-2427
s:numberOfPages
5