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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F12%3A%230000904%21RIV13-MZE-00027162
rdf:type
n3:Vysledek skos:Concept
dcterms:description
Data on the frequency of aneuploidy in farm animals are lacking and there is the need for a reliable technique which is capable of detecting all chromosomes simultaneously in a single cell. With the employment of comparative genomic hybridization coupled with the whole genome amplification technique, this study brings new information regarding the aneuploidy of individual chromosomes in pigs. Focus is directed on in vivo porcine blastocysts and late morulas, 4.7% of which were found to carry chromosomal abnormality. Further, ploidy abnormalities were examined using FISH in a sample of porcine embryos. True polyploidy was relatively rare (1.6%), whilst mixoploidy was presented in 46.8% of embryos, however it was restricted to only a small number of cells per embryo. The combined data indicates that aneuploidy is not a prevalent cause of embryo mortality in pigs. Data on the frequency of aneuploidy in farm animals are lacking and there is the need for a reliable technique which is capable of detecting all chromosomes simultaneously in a single cell. With the employment of comparative genomic hybridization coupled with the whole genome amplification technique, this study brings new information regarding the aneuploidy of individual chromosomes in pigs. Focus is directed on in vivo porcine blastocysts and late morulas, 4.7% of which were found to carry chromosomal abnormality. Further, ploidy abnormalities were examined using FISH in a sample of porcine embryos. True polyploidy was relatively rare (1.6%), whilst mixoploidy was presented in 46.8% of embryos, however it was restricted to only a small number of cells per embryo. The combined data indicates that aneuploidy is not a prevalent cause of embryo mortality in pigs.
dcterms:title
Aneuploidy Detection in Pigs Using Comparative Genomic Hybridization: From the Oocytes to Blastocysts Aneuploidy Detection in Pigs Using Comparative Genomic Hybridization: From the Oocytes to Blastocysts
skos:prefLabel
Aneuploidy Detection in Pigs Using Comparative Genomic Hybridization: From the Oocytes to Blastocysts Aneuploidy Detection in Pigs Using Comparative Genomic Hybridization: From the Oocytes to Blastocysts
skos:notation
RIV/00027162:_____/12:#0000904!RIV13-MZE-00027162
n3:predkladatel
n18:ico%3A00027162
n4:aktivita
n6:P
n4:aktivity
P(ED1.1.00/02.0068), P(QH81068)
n4:cisloPeriodika
1
n4:dodaniDat
n16:2013
n4:domaciTvurceVysledku
n13:1769111 n13:8164703 n13:8143102 n13:3647706
n4:druhVysledku
n8:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n19:predkladatel
n4:idSjednocenehoVysledku
122768
n4:idVysledku
RIV/00027162:_____/12:#0000904
n4:jazykVysledku
n11:eng
n4:klicovaSlova
IN-SITU HYBRIDIZATION; INNER CELL MASS; CHROMOSOMAL-ABNORMALITIES; PORCINE EMBRYOS; GENETIC-ANALYSIS; VITRO; GILTS; SPERM; DAY-6; STAGE
n4:klicoveSlovo
n5:CHROMOSOMAL-ABNORMALITIES n5:GENETIC-ANALYSIS n5:INNER%20CELL%20MASS n5:IN-SITU%20HYBRIDIZATION n5:GILTS n5:PORCINE%20EMBRYOS n5:DAY-6 n5:VITRO n5:SPERM n5:STAGE
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[65ED68D1A883]
n4:nazevZdroje
Plos One
n4:obor
n14:EB
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
5
n4:projekt
n9:ED1.1.00%2F02.0068 n9:QH81068
n4:rokUplatneniVysledku
n16:2012
n4:svazekPeriodika
7
n4:tvurceVysledku
Horňák, Miroslav Oráčová, Eva Rubeš, Jiří Hulínská, Pavlína Urbánková, L.
n4:wos
000301570600032
s:issn
1932-6203
s:numberOfPages
6
n15:doi
10.1371/journal.pone.0030335