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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F12%3A%230000882%21RIV13-MZE-00027162
rdf:type
skos:Concept n10:Vysledek
dcterms:description
Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of paratuberculosis in ruminants, has a lipid-rich cell wall which facilitates its survival and persistence in the environment. This property of the organism is exploited when it is cultured as decontaminating agents and antibiotics are used to suppress the growth of contaminating microflora, but such treatments can also negatively affect the isolation of MAP itself. The objective of this study was to assess the effect of the 'VAN' antibiotics (vancomycin, amphotericin B and nalidixic acid) on the viability of MAP using a propidium monoazide real time quantitative PCR (PMA qPCR) and culture. Long-term (5 week) treatment with VAN antibiotics resulted in a larger decrease in bacterial numbers compared to short-term (3 day) exposure. The PMA qPCR assay indicated that 50 mu g/mL of vancomycin, 50 mu g/mL of nalidixic acid, and 200 mu g/mL of amphotericin B were 'threshold' concentrations, respectively, above which the decline in the viability of MAP was statistically significant. Using culture, these threshold concentrations were 100 mu g/mL of vancomycin, 50-100 mu g/mL of nalidixic acid, and 100 mu g/mL of amphotericin B, respectively. Given that the two methods were found to be comparable, the PMA qPCR is a potentially more convenient and effective alternative to culture in detecting MAP. Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of paratuberculosis in ruminants, has a lipid-rich cell wall which facilitates its survival and persistence in the environment. This property of the organism is exploited when it is cultured as decontaminating agents and antibiotics are used to suppress the growth of contaminating microflora, but such treatments can also negatively affect the isolation of MAP itself. The objective of this study was to assess the effect of the 'VAN' antibiotics (vancomycin, amphotericin B and nalidixic acid) on the viability of MAP using a propidium monoazide real time quantitative PCR (PMA qPCR) and culture. Long-term (5 week) treatment with VAN antibiotics resulted in a larger decrease in bacterial numbers compared to short-term (3 day) exposure. The PMA qPCR assay indicated that 50 mu g/mL of vancomycin, 50 mu g/mL of nalidixic acid, and 200 mu g/mL of amphotericin B were 'threshold' concentrations, respectively, above which the decline in the viability of MAP was statistically significant. Using culture, these threshold concentrations were 100 mu g/mL of vancomycin, 50-100 mu g/mL of nalidixic acid, and 100 mu g/mL of amphotericin B, respectively. Given that the two methods were found to be comparable, the PMA qPCR is a potentially more convenient and effective alternative to culture in detecting MAP.
dcterms:title
Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture
skos:prefLabel
Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture
skos:notation
RIV/00027162:_____/12:#0000882!RIV13-MZE-00027162
n10:predkladatel
n18:ico%3A00027162
n3:aktivita
n9:Z n9:P
n3:aktivity
P(ED0006/01/01), P(QH81065), Z(MZE0002716202)
n3:cisloPeriodika
3
n3:dodaniDat
n13:2013
n3:domaciTvurceVysledku
n17:6439802 n17:5144523 n17:3036634 n17:3608026
n3:druhVysledku
n16:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
133250
n3:idVysledku
RIV/00027162:_____/12:#0000882
n3:jazykVysledku
n20:eng
n3:klicovaSlova
Mycobacterium avium subsp paratuberculosis; PMA qPCR; Culture; Viability; Paratuberculosis; In vitro
n3:klicoveSlovo
n6:PMA%20qPCR n6:Viability n6:Culture n6:Paratuberculosis n6:Mycobacterium%20avium%20subsp%20paratuberculosis n6:In%20vitro
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[910486CDA028]
n3:nazevZdroje
Veterinary Journal
n3:obor
n19:GJ
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:projekt
n8:ED0006%2F01%2F01 n8:QH81065
n3:rokUplatneniVysledku
n13:2012
n3:svazekPeriodika
194
n3:tvurceVysledku
Kubíčková, L. Králík, Petr Pavlík, Ivo Babák, Vladimír Přibylová, Radka
n3:wos
000313224300021
n3:zamer
n14:MZE0002716202
s:issn
1090-0233
s:numberOfPages
7
n7:doi
10.1016/j.tvjl.2012.05.002