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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F11%3A%230000847%21RIV12-MZE-00027162
rdf:type
n12:Vysledek skos:Concept
dcterms:description
Sperm plasma membrane integrity is commonly evaluated by intravital staining tests or by a test of membrane tolerance and resistance to hypo-osmotic conditions (HOS test). We proved that despite good sperm viability as detected by a simple HOS test, the degree of membrane resistance can be low. The difference in sperm survival rates during the 120-min test between groups of boar ejaculates containing higher and lower percentages of HOS-positive and eosin-negative sperm was statistically significant even though the other parameters of sperm analysis did not significantly differ. Moreover a higher percentage of sperm was recovered after thawing of bull ejaculates with a higher percentage of HOS-positive and eosin-negative sperm. The HOS test in combination with intravital staining is a procedure providing additional information concerning dynamic changes in the membrane resistance. It allows assessment of semen quality from the perspective of its further use, e.g. cryopreservation. Sperm plasma membrane integrity is commonly evaluated by intravital staining tests or by a test of membrane tolerance and resistance to hypo-osmotic conditions (HOS test). We proved that despite good sperm viability as detected by a simple HOS test, the degree of membrane resistance can be low. The difference in sperm survival rates during the 120-min test between groups of boar ejaculates containing higher and lower percentages of HOS-positive and eosin-negative sperm was statistically significant even though the other parameters of sperm analysis did not significantly differ. Moreover a higher percentage of sperm was recovered after thawing of bull ejaculates with a higher percentage of HOS-positive and eosin-negative sperm. The HOS test in combination with intravital staining is a procedure providing additional information concerning dynamic changes in the membrane resistance. It allows assessment of semen quality from the perspective of its further use, e.g. cryopreservation.
dcterms:title
Evaluation of sperm membrane resistance in boars and bulls by the modified HOS test Evaluation of sperm membrane resistance in boars and bulls by the modified HOS test
skos:prefLabel
Evaluation of sperm membrane resistance in boars and bulls by the modified HOS test Evaluation of sperm membrane resistance in boars and bulls by the modified HOS test
skos:notation
RIV/00027162:_____/11:#0000847!RIV12-MZE-00027162
n12:predkladatel
n16:ico%3A00027162
n3:aktivita
n6:Z
n3:aktivity
Z(MZE0002716202)
n3:dodaniDat
n13:2012
n3:domaciTvurceVysledku
n8:6821413 n8:7030460 n8:6098525 n8:9211152 n8:9185682
n3:druhVysledku
n17:O
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
198362
n3:idVysledku
RIV/00027162:_____/11:#0000847
n3:jazykVysledku
n11:eng
n3:klicovaSlova
sperm; HOS test; membrane resistance; bull; boar
n3:klicoveSlovo
n5:membrane%20resistance n5:HOS%20test n5:sperm n5:bull n5:boar
n3:kontrolniKodProRIV
[661C4C27AD74]
n3:obor
n15:GJ
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:rokUplatneniVysledku
n13:2011
n3:tvurceVysledku
Věžník, Zdeněk Přinosilová, Petra Hlavicová, Jaroslava Kopecká, Věra Zajícová, Atanáska
n3:zamer
n14:MZE0002716202