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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F11%3A%230000770%21RIV12-GA0-00027162
rdf:type
skos:Concept n6:Vysledek
dcterms:description
Porcine sapoviruse have been considered as an aethiological agent of gastroenteritis in pigs. In this study, we therefore analysed 251 faecal samples obtained from diarrhoeic pigs in the Czech Republic during January 2005-June 2010 and tested them by negative staining electron microscopy for the presence of sapoviruses. Only one sample showed the presence of viral particles with characteristic sapovirus morphology. Presence of sapovirus was confirmed by RT-PCR assay with primers specific to sapoviral RNA polymerase gene and capsid gene. The phylogenetic analysis based on partial RNA polymerase sequence placed the new Czech isolate into the GVII genogroup of porcine sapoviruses, however, analysis of partial capsid gene sequence classified isolate as GIII of the genus Sapovirus. The findings of phylogenetic analysis indicate, that recombinant porcine sapovirus was identified. According to our knowledge this is the first description of porcine sapovirus in domestic pigs in the Czech Republic. Porcine sapoviruse have been considered as an aethiological agent of gastroenteritis in pigs. In this study, we therefore analysed 251 faecal samples obtained from diarrhoeic pigs in the Czech Republic during January 2005-June 2010 and tested them by negative staining electron microscopy for the presence of sapoviruses. Only one sample showed the presence of viral particles with characteristic sapovirus morphology. Presence of sapovirus was confirmed by RT-PCR assay with primers specific to sapoviral RNA polymerase gene and capsid gene. The phylogenetic analysis based on partial RNA polymerase sequence placed the new Czech isolate into the GVII genogroup of porcine sapoviruses, however, analysis of partial capsid gene sequence classified isolate as GIII of the genus Sapovirus. The findings of phylogenetic analysis indicate, that recombinant porcine sapovirus was identified. According to our knowledge this is the first description of porcine sapovirus in domestic pigs in the Czech Republic.
dcterms:title
Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report
skos:prefLabel
Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report
skos:notation
RIV/00027162:_____/11:#0000770!RIV12-GA0-00027162
n6:predkladatel
n7:ico%3A00027162
n3:aktivita
n4:P
n3:aktivity
P(ED0006/01/01), P(GP524/09/P195)
n3:cisloPeriodika
8
n3:dodaniDat
n16:2012
n3:domaciTvurceVysledku
n15:4214064 n15:2432749 n15:6416691
n3:druhVysledku
n8:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
213479
n3:idVysledku
RIV/00027162:_____/11:#0000770
n3:jazykVysledku
n17:eng
n3:klicovaSlova
calicivirus; RT-PCR; phylogenetic analysis; genogroup; enteritis
n3:klicoveSlovo
n10:genogroup n10:enteritis n10:RT-PCR n10:phylogenetic%20analysis n10:calicivirus
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[733E27B6C98D]
n3:nazevZdroje
Veterinární Medicína
n3:obor
n12:GJ
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n13:GP524%2F09%2FP195 n13:ED0006%2F01%2F01
n3:rokUplatneniVysledku
n16:2011
n3:svazekPeriodika
56
n3:tvurceVysledku
Dufková, Lucie Kulich, Pavel Prodělalová, Jana
n3:wos
000295252600007
s:issn
0375-8427
s:numberOfPages
7