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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F11%3A%230000743%21RIV12-MZE-00027162
rdf:type
skos:Concept n15:Vysledek
dcterms:description
This study focused on the development of a DNA isolation procedure for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) in faeces by IS900 and F57 quantitative real time PCR (qPCR) and their comparison with culture. The recovery of MAP DNA ranged from 29.1 to 102.4% of the input amount of MAP with median 37.9%. The limit of detection was determined to be 1.03 x 10(4) for F57 qPCR and 6.87 x 10(2) MAP cells per gram of faeces for IS900 qPCR, respectively. The developed technique for DNA isolation was coupled with 15900 qPCR and compared to traditional MAP culture using a cohort of 1906 faecal samples examined from 12 dairy cattle farms in our laboratory. From those 1906 original faecal samples. 875 were positive by IS900 qPCR and 169 by culture. This data facilitated development of a predictive model capable of estimating the probability of being culture positive by estimating the absolute number of MAP per gram of faeces as determined IS900 qPCR without performing the culture. This study focused on the development of a DNA isolation procedure for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) in faeces by IS900 and F57 quantitative real time PCR (qPCR) and their comparison with culture. The recovery of MAP DNA ranged from 29.1 to 102.4% of the input amount of MAP with median 37.9%. The limit of detection was determined to be 1.03 x 10(4) for F57 qPCR and 6.87 x 10(2) MAP cells per gram of faeces for IS900 qPCR, respectively. The developed technique for DNA isolation was coupled with 15900 qPCR and compared to traditional MAP culture using a cohort of 1906 faecal samples examined from 12 dairy cattle farms in our laboratory. From those 1906 original faecal samples. 875 were positive by IS900 qPCR and 169 by culture. This data facilitated development of a predictive model capable of estimating the probability of being culture positive by estimating the absolute number of MAP per gram of faeces as determined IS900 qPCR without performing the culture.
dcterms:title
Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR
skos:prefLabel
Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR
skos:notation
RIV/00027162:_____/11:#0000743!RIV12-MZE-00027162
n15:predkladatel
n16:ico%3A00027162
n3:aktivita
n11:P n11:Z
n3:aktivity
P(ED0006/01/01), P(QH81065), Z(MZE0002716202)
n3:cisloPeriodika
1-2
n3:dodaniDat
n10:2012
n3:domaciTvurceVysledku
n6:5144523 n6:6439802 n6:9537007 n6:6982476 n6:3608026
n3:druhVysledku
n14:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
194022
n3:idVysledku
RIV/00027162:_____/11:#0000743
n3:jazykVysledku
n4:eng
n3:klicovaSlova
MAP; Johne's disease; Faeces DNA isolation; Paratuberculosis; qPCR
n3:klicoveSlovo
n5:Faeces%20DNA%20isolation n5:MAP n5:Paratuberculosis n5:Johne%27s%20disease n5:qPCR
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[FA4E33EB21E6]
n3:nazevZdroje
Veterinary Microbiology
n3:obor
n7:EE
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n12:QH81065 n12:ED0006%2F01%2F01
n3:rokUplatneniVysledku
n10:2011
n3:svazekPeriodika
149
n3:tvurceVysledku
Pavlík, Ivo Králová, Alena Slaná, Iva Králík, Petr Babák, Vladimír Whitlock, R. H.
n3:wos
000289031600016
n3:zamer
n19:MZE0002716202
s:issn
0378-1135
s:numberOfPages
6