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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F10%3A%230000695%21RIV11-MZE-00027162
rdf:type
skos:Concept n18:Vysledek
dcterms:description
The aim of the present study was to study by culture, and a quantitative real-time polymerase chain reaction, the presence of bacteria in consecutive semen, blood, and fecal samples collected from one infected Piedmont breeding bull during a 380 day period. Five out of seven blood samples and all nine semen samples were positive on real-time quantitative polymerase chain reaction with 101 to 102 and 102 to 104 copies of IS900/F57 per ml, respectively. In all, there were 9 fecal culture positive samples with too numerous to count colony forming units and positive real-time quantitiative polymerase chain reaction ranging from 105 to 107 copies of IS900/F57. After the bull was euthanized, Mycobacterium avium subsp. paratuberculosis was cultured from various parts of small and large intestines, liver tissue, lymph nodes, and from epididymis and vesicular glands. The aim of the present study was to study by culture, and a quantitative real-time polymerase chain reaction, the presence of bacteria in consecutive semen, blood, and fecal samples collected from one infected Piedmont breeding bull during a 380 day period. Five out of seven blood samples and all nine semen samples were positive on real-time quantitative polymerase chain reaction with 101 to 102 and 102 to 104 copies of IS900/F57 per ml, respectively. In all, there were 9 fecal culture positive samples with too numerous to count colony forming units and positive real-time quantitiative polymerase chain reaction ranging from 105 to 107 copies of IS900/F57. After the bull was euthanized, Mycobacterium avium subsp. paratuberculosis was cultured from various parts of small and large intestines, liver tissue, lymph nodes, and from epididymis and vesicular glands.
dcterms:title
Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations
skos:prefLabel
Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations
skos:notation
RIV/00027162:_____/10:#0000695!RIV11-MZE-00027162
n3:aktivita
n13:Z n13:P
n3:aktivity
P(ED0006/01/01), P(QH81065), Z(MZE0002716202)
n3:cisloPeriodika
10
n3:dodaniDat
n7:2011
n3:domaciTvurceVysledku
n11:4307429 n11:6439802 n11:3608026 n11:6982476
n3:druhVysledku
n4:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
251871
n3:idVysledku
RIV/00027162:_____/10:#0000695
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Breeding bull; Cattle; Johne's disease; paratuberculosis; semen analysis
n3:klicoveSlovo
n9:Cattle n9:Johne%27s%20disease n9:paratuberculosis n9:semen%20analysis n9:Breeding%20bull
n3:kodStatuVydavatele
JP - Japonsko
n3:kontrolniKodProRIV
[C72BD71CEDB8]
n3:nazevZdroje
Journal of Veterinary Medical Science
n3:obor
n14:GJ
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
7
n3:projekt
n15:ED0006%2F01%2F01 n15:QH81065
n3:rokUplatneniVysledku
n7:2010
n3:svazekPeriodika
72
n3:tvurceVysledku
Pavlík, Ivo Králík, Petr Baumgartner, W. Khol, J. L. Aurich, C. Beran, Vladimír Slaná, Iva
n3:wos
000284246900005
n3:zamer
n6:MZE0002716202
s:issn
0916-7250
s:numberOfPages
6