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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F10%3A%230000633%21RIV11-MZE-00027162
rdf:type
skos:Concept n14:Vysledek
dcterms:description
We have evaluated a promising technology combining sample treatment with propidium monoazide (PMA) prior to DNA extraction for selective detection of cells with intact cell membranes with detection of sequence element F57 by quantitative PCR (F57 qPCR). Element F57 is unique for MAP and is not known to exist in any other bacterial species. Conditions of PMA treatment were optimised for MAP isolate 7082 using live and heat-killed cells and comparing different DNA extraction procedures. The subsequent successful application of the optimised protocol to four other MAP isolates of different origins suggested that the optimised protocol might be broadly applicable to different MAP strains. Furthermore, different equations were compared to use the data resulting from this technology to optimally predict the percentage of live MAP cells in mixtures containing both live and dead cells. We have evaluated a promising technology combining sample treatment with propidium monoazide (PMA) prior to DNA extraction for selective detection of cells with intact cell membranes with detection of sequence element F57 by quantitative PCR (F57 qPCR). Element F57 is unique for MAP and is not known to exist in any other bacterial species. Conditions of PMA treatment were optimised for MAP isolate 7082 using live and heat-killed cells and comparing different DNA extraction procedures. The subsequent successful application of the optimised protocol to four other MAP isolates of different origins suggested that the optimised protocol might be broadly applicable to different MAP strains. Furthermore, different equations were compared to use the data resulting from this technology to optimally predict the percentage of live MAP cells in mixtures containing both live and dead cells.
dcterms:title
Mycobacterium avium subsp paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment Mycobacterium avium subsp paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment
skos:prefLabel
Mycobacterium avium subsp paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment Mycobacterium avium subsp paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment
skos:notation
RIV/00027162:_____/10:#0000633!RIV11-MZE-00027162
n5:aktivita
n12:P n12:Z
n5:aktivity
P(ED0006/01/01), P(QH81065), Z(MZE0002716202)
n5:cisloPeriodika
Suppl 1
n5:dodaniDat
n10:2011
n5:domaciTvurceVysledku
n7:3608026 n7:6439802
n5:druhVysledku
n18:J
n5:duvernostUdaju
n15:S
n5:entitaPredkladatele
n13:predkladatel
n5:idSjednocenehoVysledku
273323
n5:idVysledku
RIV/00027162:_____/10:#0000633
n5:jazykVysledku
n17:eng
n5:klicovaSlova
MAP; Johne's disease; PMA; Paratuberculosis; Crohn's disease
n5:klicoveSlovo
n8:PMA n8:Johne%27s%20disease n8:Crohn%27s%20disease n8:Paratuberculosis n8:MAP
n5:kodStatuVydavatele
NL - Nizozemsko
n5:kontrolniKodProRIV
[81788D5FA23C]
n5:nazevZdroje
International Journal of Food Microbiology
n5:obor
n16:GJ
n5:pocetDomacichTvurcuVysledku
2
n5:pocetTvurcuVysledku
3
n5:projekt
n9:QH81065 n9:ED0006%2F01%2F01
n5:rokUplatneniVysledku
n10:2010
n5:svazekPeriodika
141
n5:tvurceVysledku
Pavlík, Ivo Králík, Petr Nocker, A.
n5:wos
000281830700010
n5:zamer
n6:MZE0002716202
s:issn
0168-1605
s:numberOfPages
7