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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F10%3A%230000630%21RIV11-MZE-00027162
rdf:type
n9:Vysledek skos:Concept
dcterms:description
In this study three different methods (quantitative real time PCR, combined phage IS900 PCR and conventional cultivation) were used to detect the presence of MAP in bulk tank milk (BTM) and cheese originating from sheep, goat and mixed milks from farms and products in Cyprus. During the first survey the presence of MAP was detected in 63 (28.6%) of cows' BTM samples by quantitative real time PCR. A second survey of BTM used a new combined phage IS900 PCR assay, and in this case MAP was detected in 50 (22.2%) samples showing a good level of agreement by both methods. The two isolates recovered from BTM were identified by IS1311 PCR REA as cattle and sheep strains, respectively. In contrast when cheese samples were tested, MAP DNA was detected by quantitative real time PCR in seven (25.0%) samples (n=28). However no viable MAP was detected when either the combined phage IS900 PCR or conventional culture methods were used. In this study three different methods (quantitative real time PCR, combined phage IS900 PCR and conventional cultivation) were used to detect the presence of MAP in bulk tank milk (BTM) and cheese originating from sheep, goat and mixed milks from farms and products in Cyprus. During the first survey the presence of MAP was detected in 63 (28.6%) of cows' BTM samples by quantitative real time PCR. A second survey of BTM used a new combined phage IS900 PCR assay, and in this case MAP was detected in 50 (22.2%) samples showing a good level of agreement by both methods. The two isolates recovered from BTM were identified by IS1311 PCR REA as cattle and sheep strains, respectively. In contrast when cheese samples were tested, MAP DNA was detected by quantitative real time PCR in seven (25.0%) samples (n=28). However no viable MAP was detected when either the combined phage IS900 PCR or conventional culture methods were used.
dcterms:title
Rapid detection methods for viable Mycobacterium avium subspecies paratuberculosis in milk and cheese Rapid detection methods for viable Mycobacterium avium subspecies paratuberculosis in milk and cheese
skos:prefLabel
Rapid detection methods for viable Mycobacterium avium subspecies paratuberculosis in milk and cheese Rapid detection methods for viable Mycobacterium avium subspecies paratuberculosis in milk and cheese
skos:notation
RIV/00027162:_____/10:#0000630!RIV11-MZE-00027162
n4:aktivita
n12:Z
n4:aktivity
Z(MZE0002716202)
n4:cisloPeriodika
Suppl. 1
n4:dodaniDat
n5:2011
n4:domaciTvurceVysledku
n16:6439802 n16:6982476
n4:druhVysledku
n7:J
n4:duvernostUdaju
n10:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
283913
n4:idVysledku
RIV/00027162:_____/10:#0000630
n4:jazykVysledku
n15:eng
n4:klicovaSlova
milk; culture; PCR; decontamination; food safety; real time PCR
n4:klicoveSlovo
n13:culture n13:PCR n13:food%20safety n13:real%20time%20PCR n13:decontamination n13:milk
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[C3F0B68CD253]
n4:nazevZdroje
International Journal of Food Microbiology
n4:obor
n11:GJ
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
7
n4:rokUplatneniVysledku
n5:2010
n4:svazekPeriodika
141
n4:tvurceVysledku
Liapi, M. Botsaris, G. Rees, C. Pavlík, Ivo Economides, C. Slaná, Iva Dodd, C.
n4:wos
000281830700011
n4:zamer
n6:MZE0002716202
s:issn
0168-1605
s:numberOfPages
4