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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F09%3A%230000551%21RIV10-MZE-00027162
rdf:type
skos:Concept n17:Vysledek
dcterms:description
This study aimed at examining the interaction between mycotoxin deoxynivalenol (DON) and Salmonella Typhimurium at the level of the porcine innate immune system, represented by macrophages. First, we assessed the direct cytotoxic effect of DON on porcine macrophages. Incubation with 0.25 ug/ml of DON or higher resulted in a significant cytotoxic effect after 24 h of incubation. Secondly, the direct toxic effect of DON on the growth and on the expression of Salmonella pathogenicity island 1 (SPI-1) and SPI-2 virulence genes of S. Typhimurium was determined. At low non-cytotoxic concentrations, DON was of no effect on growth or virulence gene expression of S. Typhimurium. However, when the invasion and intracellular survival of S. Typhimurium in macrophages preexposed to 0.025 ug/ml of DON was examined, DON significantly promoted the uptake of S. Typhimurium into macrophages which coincided with F-actin reorganization due to the activation of ERK1/2. This study aimed at examining the interaction between mycotoxin deoxynivalenol (DON) and Salmonella Typhimurium at the level of the porcine innate immune system, represented by macrophages. First, we assessed the direct cytotoxic effect of DON on porcine macrophages. Incubation with 0.25 ug/ml of DON or higher resulted in a significant cytotoxic effect after 24 h of incubation. Secondly, the direct toxic effect of DON on the growth and on the expression of Salmonella pathogenicity island 1 (SPI-1) and SPI-2 virulence genes of S. Typhimurium was determined. At low non-cytotoxic concentrations, DON was of no effect on growth or virulence gene expression of S. Typhimurium. However, when the invasion and intracellular survival of S. Typhimurium in macrophages preexposed to 0.025 ug/ml of DON was examined, DON significantly promoted the uptake of S. Typhimurium into macrophages which coincided with F-actin reorganization due to the activation of ERK1/2.
dcterms:title
The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK 1/2 induced cytoskeleton reorganization The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK 1/2 induced cytoskeleton reorganization
skos:prefLabel
The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK 1/2 induced cytoskeleton reorganization The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK 1/2 induced cytoskeleton reorganization
skos:notation
RIV/00027162:_____/09:#0000551!RIV10-MZE-00027162
n3:aktivita
n7:Z
n3:aktivity
Z(MZE0002716201)
n3:cisloPeriodika
6
n3:dodaniDat
n11:2010
n3:domaciTvurceVysledku
n13:9231625
n3:druhVysledku
n16:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
328159
n3:idVysledku
RIV/00027162:_____/09:#0000551
n3:jazykVysledku
n15:eng
n3:klicovaSlova
deoxynivalenol; Salmonella Typhimurium; pig; macrophage; cytoskeleton
n3:klicoveSlovo
n6:macrophage n6:deoxynivalenol n6:cytoskeleton n6:Salmonella%20Typhimurium n6:pig
n3:kodStatuVydavatele
FR - Francouzská republika
n3:kontrolniKodProRIV
[5B6EB937EEF4]
n3:nazevZdroje
Veterinary Research
n3:obor
n5:GJ
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
8
n3:rokUplatneniVysledku
n11:2009
n3:svazekPeriodika
40
n3:tvurceVysledku
Ducatelle, R. Boyen, F. Pasmans, F. De Backer, P. Croubels, S. Haesebrouck, F. Rychlík, Ivan Vandenbroucke, V.
n3:wos
000272039500013
n3:zamer
n14:MZE0002716201
s:issn
0928-4249
s:numberOfPages
12