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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F09%3A%230000496%21RIV10-MZE-00027162
rdf:type
n7:Vysledek skos:Concept
dcterms:description
The aim of the study was retyping and reclassification of the 27 strains of porcine enteroviruses deposited in the Collection of Animal Pathogenic Microorganisms. Viral strains were isolated over the period 1960-1980 predominantly from pigs with encephalomyelitis. Twenty-four of 27 isolates were classified as porcine enterovirus serotype 1 based on physicochemicals properties of their virions and growth characteristics. The viral strains were reclassified using RT-PCR protocol that allowed detection of the genus Teschovirus (PTV) and the Porcine teschovirus serotype 1 (PTV-1). The fragment characteristic for PTV was detected in all samples. The fragment characteristic for PTV-1 was detected with the exception of three strains. Used RT-PCR method was subsequently applied to the detection of PTV in pig fecal samples. Ten of 22 faecal samples were found to be PTV positive, however none of 22 samples generated amplicon specific for the PTV-1. The aim of the study was retyping and reclassification of the 27 strains of porcine enteroviruses deposited in the Collection of Animal Pathogenic Microorganisms. Viral strains were isolated over the period 1960-1980 predominantly from pigs with encephalomyelitis. Twenty-four of 27 isolates were classified as porcine enterovirus serotype 1 based on physicochemicals properties of their virions and growth characteristics. The viral strains were reclassified using RT-PCR protocol that allowed detection of the genus Teschovirus (PTV) and the Porcine teschovirus serotype 1 (PTV-1). The fragment characteristic for PTV was detected in all samples. The fragment characteristic for PTV-1 was detected with the exception of three strains. Used RT-PCR method was subsequently applied to the detection of PTV in pig fecal samples. Ten of 22 faecal samples were found to be PTV positive, however none of 22 samples generated amplicon specific for the PTV-1.
dcterms:title
Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea
skos:prefLabel
Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea
skos:notation
RIV/00027162:_____/09:#0000496!RIV10-MZE-00027162
n3:aktivita
n11:Z
n3:aktivity
Z(MZE0002716202)
n3:cisloPeriodika
10
n3:dodaniDat
n9:2010
n3:domaciTvurceVysledku
n8:7925158 n8:6416691 n8:1422707
n3:druhVysledku
n14:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
316222
n3:idVysledku
RIV/00027162:_____/09:#0000496
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Teschovirus; PTV-1; fecal samples; reverse transcription; polymerase chain reaction
n3:klicoveSlovo
n6:Teschovirus n6:fecal%20samples n6:reverse%20transcription n6:PTV-1 n6:polymerase%20chain%20reaction
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[7A6054E89311]
n3:nazevZdroje
Veterinární medicína
n3:obor
n4:GJ
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n9:2009
n3:svazekPeriodika
54
n3:tvurceVysledku
Valíček, Lubomír Malenovská, Hana Prodělalová, Jana
n3:wos
000272392800002
n3:zamer
n13:MZE0002716202
s:issn
0375-8427
s:numberOfPages
6