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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F09%3A%230000483%21RIV10-MZE-00027162
rdf:type
n5:Vysledek skos:Concept
dcterms:description
The detection of IS901 restriction fragment length polymorphism (RFLP) types of Mycobacterium avium subsp. avium (MAA) isolates from different domestic animals between 1998 and 2004 was the aim of this study. Detected RFLP types were compared with previously described RFLP types (the database of the OIE Reference Laboratory for Avian Tuberculosis, Brno, Czech Republic). A total of 62 isolates (56 from pigs, 5 from poultry and one from cattle) were identified with IS901 PCR and IS901 RFLP typed using PvuII and PstI endonucleases. Seven PvuII and 11 PstI RFLP types resulted in 12 combined PvuII PstI types; none of these matched the combined RFLP types described in previous studies. Two new PvuII and eight new PstI RFLP types were described. Identical RFLP types were found among isolates originating from individual farms. A unique RFLP type F-A17 was detected in isolates from different pig herds and also in poultry isolates. Detection of identical RFLP types on different farms may reflect one MAA source. The detection of IS901 restriction fragment length polymorphism (RFLP) types of Mycobacterium avium subsp. avium (MAA) isolates from different domestic animals between 1998 and 2004 was the aim of this study. Detected RFLP types were compared with previously described RFLP types (the database of the OIE Reference Laboratory for Avian Tuberculosis, Brno, Czech Republic). A total of 62 isolates (56 from pigs, 5 from poultry and one from cattle) were identified with IS901 PCR and IS901 RFLP typed using PvuII and PstI endonucleases. Seven PvuII and 11 PstI RFLP types resulted in 12 combined PvuII PstI types; none of these matched the combined RFLP types described in previous studies. Two new PvuII and eight new PstI RFLP types were described. Identical RFLP types were found among isolates originating from individual farms. A unique RFLP type F-A17 was detected in isolates from different pig herds and also in poultry isolates. Detection of identical RFLP types on different farms may reflect one MAA source.
dcterms:title
Genotyping of Mycobacterium avium subsp avium isolates from domestic animals in Slovenia by IS901 RFLP Genotyping of Mycobacterium avium subsp avium isolates from domestic animals in Slovenia by IS901 RFLP
skos:prefLabel
Genotyping of Mycobacterium avium subsp avium isolates from domestic animals in Slovenia by IS901 RFLP Genotyping of Mycobacterium avium subsp avium isolates from domestic animals in Slovenia by IS901 RFLP
skos:notation
RIV/00027162:_____/09:#0000483!RIV10-MZE-00027162
n3:aktivita
n8:Z
n3:aktivity
Z(MZE0002716202)
n3:cisloPeriodika
6
n3:dodaniDat
n4:2010
n3:domaciTvurceVysledku
n6:1295888 n6:6439802
n3:druhVysledku
n15:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
316221
n3:idVysledku
RIV/00027162:_____/09:#0000483
n3:jazykVysledku
n14:eng
n3:klicovaSlova
genotyping; mycobacteriosis; avian tuberculosis; molecular epidemiology; zoonosis; database for IS901 RFLP types
n3:klicoveSlovo
n11:database%20for%20IS901%20RFLP%20types n11:mycobacteriosis n11:molecular%20epidemiology n11:genotyping n11:zoonosis n11:avian%20tuberculosis
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[85FCDDECA69A]
n3:nazevZdroje
Veterinární medicína
n3:obor
n17:GJ
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
5
n3:rokUplatneniVysledku
n4:2009
n3:svazekPeriodika
54
n3:tvurceVysledku
Pavlík, Ivo Ocepek, M. Krt, B. Pate, M. Morávková, Monika
n3:wos
000268784300002
n3:zamer
n12:MZE0002716202
s:issn
0375-8427
s:numberOfPages
10