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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F08%3A%230000423%21RIV09-MZE-00027162
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Centromeric repetitive sequences from chromosome 4 (CCR4/10sat) of Cricetus cricetus (CCR, Cricetidae, Rodentia) were isolated using the laser microdissection, followed by DOP-PCR amplification and labelling. The probe was mapped by fluorescent in situ hybridisation onto C. cricetus and another member of Cricetidae, Peromyscus eremicus. The centromeric sequences showed to be present in another C. cricetus chromosome (CCR10) besides CCR4. Moreover, these almost chromosome-specific sequences revealed to be present in the P. eremicus genome, and most interestingly, displaying a ubiquitous scattered distribution throughout this karyotype. In both species, a co-localisation of CCR4/10sat with constitutive heterochromatin was found. The presence of these orthologous sequences in both genomes is suggestive of a phylogenetic proximity. Centromerické repetitivní sekvence z chromozomu 4 (CCR4/10sat) Cricetus cricetus (CCR, Cricetidae, Rodentia) byly izolovány laserovou mikrodisekcí, amplifikovány pomocí DOP-PCR a značeny. Sonda byla mapována fluorescenční in situ hybridizací na chromozomy C. cricetus a dalšího zástupce čeledi Cricetidae, Peromyscus eremicus. Centromerické sekvence byly kromě CCR4 přítomny i na dalším chromozomu (CCR10) C. cricetus. U P. eremicus sonda vytvářela rozptýlený signálna všech chromozomech. U obou druhů byla nalezena společná lokalizace repetitivní CCR4/10sat s konstitutivním heterochromatinem. Přítomnost této orthologní sekvence u obou genomů naznačuje jejich fylogentickou příbuznost. Centromeric repetitive sequences from chromosome 4 (CCR4/10sat) of Cricetus cricetus (CCR, Cricetidae, Rodentia) were isolated using the laser microdissection, followed by DOP-PCR amplification and labelling. The probe was mapped by fluorescent in situ hybridisation onto C. cricetus and another member of Cricetidae, Peromyscus eremicus. The centromeric sequences showed to be present in another C. cricetus chromosome (CCR10) besides CCR4. Moreover, these almost chromosome-specific sequences revealed to be present in the P. eremicus genome, and most interestingly, displaying a ubiquitous scattered distribution throughout this karyotype. In both species, a co-localisation of CCR4/10sat with constitutive heterochromatin was found. The presence of these orthologous sequences in both genomes is suggestive of a phylogenetic proximity.
dcterms:title
Different evolutionary trails in the related genomes Cricetus cricetus and Peromyscus eremicus (Rodentia, Cricetidae) uncovered by orthologous satellite DNA repositioning Different evolutionary trails in the related genomes Cricetus cricetus and Peromyscus eremicus (Rodentia, Cricetidae) uncovered by orthologous satellite DNA repositioning Různé cesty evoluce u příbuzných druhů Cricetus cricetus and Peromyscus eremicus (Rodentia, Cricetidae) stanovené přesunem orthologní satelitní DNA
skos:prefLabel
Různé cesty evoluce u příbuzných druhů Cricetus cricetus and Peromyscus eremicus (Rodentia, Cricetidae) stanovené přesunem orthologní satelitní DNA Different evolutionary trails in the related genomes Cricetus cricetus and Peromyscus eremicus (Rodentia, Cricetidae) uncovered by orthologous satellite DNA repositioning Different evolutionary trails in the related genomes Cricetus cricetus and Peromyscus eremicus (Rodentia, Cricetidae) uncovered by orthologous satellite DNA repositioning
skos:notation
RIV/00027162:_____/08:#0000423!RIV09-MZE-00027162
n3:aktivita
n14:Z
n3:aktivity
Z(MZE0002716201)
n3:cisloPeriodika
8
n3:dodaniDat
n12:2009
n3:domaciTvurceVysledku
n13:8408033 n13:8164703
n3:druhVysledku
n7:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
363549
n3:idVysledku
RIV/00027162:_____/08:#0000423
n3:jazykVysledku
n5:eng
n3:klicovaSlova
Cricetus cricetus; Peromyscus eremicus; repetitive sequences; phylogenetic
n3:klicoveSlovo
n10:repetitive%20sequences n10:Cricetus%20cricetus n10:Peromyscus%20eremicus n10:phylogenetic
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[08F5372B45A0]
n3:nazevZdroje
Micron
n3:obor
n16:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
7
n3:rokUplatneniVysledku
n12:2008
n3:svazekPeriodika
39
n3:tvurceVysledku
Louzada, S. Rubeš, Jiří Chaves, R. Adega, F. Paco, A. Kubíčková, Svatava Guedes-Pinto, H.
n3:wos
000260873600010
n3:zamer
n9:MZE0002716201
s:issn
0968-4328
s:numberOfPages
7