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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F08%3A%230000406%21RIV09-MZE-00027162
rdf:type
skos:Concept n12:Vysledek
dcterms:description
An AI Ayrshire bull was subjected to cytogenetic examination due to lowered fertility. Preliminary Giemsa staining revealed a normal chromosome complement (60,XY) and G-banding did not allow us to draw a clear conclusion concerning an occurrence of chromosome rearrangement. Testicles were collected at slaughter and synaptonemal complex (SC) analysis revealed a large cross-shaped tetravalent configuration in pachytene spreads. No association between the tetravalent and XY bivalent was observed. Chromosome painting, with the use of bovine whole chromosome painting probes, conjugated with DAPI staining, facilitated a detailed description of the translocation rcp(2;4)(q45;q34). This study shows that post mortem analysis of synaptonemal complexes is a simple and useful tool for the preliminary detection of reciprocal translocation carriers. U býka Ayrshirského plemene bylo provedeno cytogenetické vyšetření z důvodu jeho snížené fertility. Po barvení Giemsou byl zjištěný normální karyotyp (60,XY) and G-pruhování neodhalilo žádnou chromozomální přestavbu. Po porážce byla odebrána varlata, analýza synaptonemálních komplexů odhalila velký tetravalent v pachytenních buňkách. Žádné spojení mezi tetravalentem a XY bivalentem nebylo pozorováno. Hybridizace s chromozom-specifickými paintingovými sondami umožnila detailní popis translokace rcp(2;4)(q45;q34). Studie ukázala, že post mortem analýza synaptonemálního kompexu je jednoduchá a užitečná metoda pro předběžnou detekci nosičů reciproké translokace. An AI Ayrshire bull was subjected to cytogenetic examination due to lowered fertility. Preliminary Giemsa staining revealed a normal chromosome complement (60,XY) and G-banding did not allow us to draw a clear conclusion concerning an occurrence of chromosome rearrangement. Testicles were collected at slaughter and synaptonemal complex (SC) analysis revealed a large cross-shaped tetravalent configuration in pachytene spreads. No association between the tetravalent and XY bivalent was observed. Chromosome painting, with the use of bovine whole chromosome painting probes, conjugated with DAPI staining, facilitated a detailed description of the translocation rcp(2;4)(q45;q34). This study shows that post mortem analysis of synaptonemal complexes is a simple and useful tool for the preliminary detection of reciprocal translocation carriers.
dcterms:title
Identification of a new reciprocal translocation Y t(2;4)(q45;q34) in an AI bull of Ayrshire breed, with the use of synaptonemal complex technique and chromosome painting Identification of a new reciprocal translocation Y t(2;4)(q45;q34) in an AI bull of Ayrshire breed, with the use of synaptonemal complex technique and chromosome painting Identifikace nové reciproké translokace Y t(2;4)(q45;q34) u býka of Ayrshirského plemene využitím analýzy synaptonemálních komplexů a paintingových sond
skos:prefLabel
Identification of a new reciprocal translocation Y t(2;4)(q45;q34) in an AI bull of Ayrshire breed, with the use of synaptonemal complex technique and chromosome painting Identifikace nové reciproké translokace Y t(2;4)(q45;q34) u býka of Ayrshirského plemene využitím analýzy synaptonemálních komplexů a paintingových sond Identification of a new reciprocal translocation Y t(2;4)(q45;q34) in an AI bull of Ayrshire breed, with the use of synaptonemal complex technique and chromosome painting
skos:notation
RIV/00027162:_____/08:#0000406!RIV09-MZE-00027162
n5:aktivita
n10:Z
n5:aktivity
Z(MZE0002716201)
n5:cisloPeriodika
3-4
n5:dodaniDat
n7:2009
n5:domaciTvurceVysledku
n15:8164703 n15:4702077
n5:druhVysledku
n14:J
n5:duvernostUdaju
n9:S
n5:entitaPredkladatele
n6:predkladatel
n5:idSjednocenehoVysledku
371197
n5:idVysledku
RIV/00027162:_____/08:#0000406
n5:jazykVysledku
n13:eng
n5:klicovaSlova
translocation; synaptonemal complex; chromosome painting; bull
n5:klicoveSlovo
n8:synaptonemal%20complex n8:chromosome%20painting n8:translocation n8:bull
n5:kodStatuVydavatele
CH - Švýcarská konfederace
n5:kontrolniKodProRIV
[92598AFE3F28]
n5:nazevZdroje
Cytogenetic and Genome Research
n5:obor
n11:EB
n5:pocetDomacichTvurcuVysledku
2
n5:pocetTvurcuVysledku
8
n5:rokUplatneniVysledku
n7:2008
n5:svazekPeriodika
121
n5:tvurceVysledku
Andersson, M. Sosnowski, J. Switonski, M. Černohorská, Halina Rubeš, Jiří Kopp, C. Nowacka-Woszuk, J. Szczerbal, I.
n5:wos
000259032000011
n5:zamer
n16:MZE0002716201
s:issn
1424-8581
s:numberOfPages
4