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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F07%3A%230000257%21RIV08-MZE-00027162
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Using laser microdissection we prepared a set of horse chromosome arm-specific probes. Most of the probes were generated from horse chromosomes, some of them were derived from Equus zebra hartmannae. The set of probes were hybridized onto E. grevyi chromosomes in order to establish a genome-wide chromosomal correspondence between this zebra and horse. The use of arm-specific probes provided us with more information on the mutual arrangement of the genomes than we could obtain by means of whole-chromosome paints generated by flow sorting, even if we used reciprocal painting with probe sets from both species. By comparison of our results and results of comparative mapping in E. burchelli, we also established the chromosomal correspondence between E. grevyi and E. burchelli, providing evidence for a very close karyotypic relationship between these two zebra species. Establishment of the comparative map for E. grevyi contributed to the knowledge of the karyotypic phylogeny in the Equidae family. Pomocí laserové mikrodisekce jsme připravili sadu sond specifických pro jednotlivá ramena chromosomů koně. Tato sada sond byla hybridizována s chromosomy zebry Grevyho za účelem určení karyotypových vztahů mezi touto zebrou a koněm. Použití sond specifických pro ramena chromosomů nám poskytlo více informací o vzájemných přestavbách genomů než bychom získali pomocí celochromosomových sond, a to i v tom případě, že bychom použili reciproční přístup se sondami obou druhů. Porovnáním našich výsledků a výsledků comparativního mapování u E. burchelli jsme rovněž určili karyotypové vztahy mezi E. grevyi a E. burchelli, které jsou velmi blízké. Vytvořením komparativní mapy zebry Grevyho jsme přispěli ke znalostem karyotypové fylogeneze u čeledi Equidae. Using laser microdissection we prepared a set of horse chromosome arm-specific probes. Most of the probes were generated from horse chromosomes, some of them were derived from Equus zebra hartmannae. The set of probes were hybridized onto E. grevyi chromosomes in order to establish a genome-wide chromosomal correspondence between this zebra and horse. The use of arm-specific probes provided us with more information on the mutual arrangement of the genomes than we could obtain by means of whole-chromosome paints generated by flow sorting, even if we used reciprocal painting with probe sets from both species. By comparison of our results and results of comparative mapping in E. burchelli, we also established the chromosomal correspondence between E. grevyi and E. burchelli, providing evidence for a very close karyotypic relationship between these two zebra species. Establishment of the comparative map for E. grevyi contributed to the knowledge of the karyotypic phylogeny in the Equidae family.
dcterms:title
Karyotypové vztahy mezi Equus grevyi, Equus burchelli a koněm domácím definované pomocí sond specifických pro ramena koňských chromosomů Karyotypic relationships among Equus grevyi, Equus burchelli and domestic horse defined using horse chromosome arm-specific probes Karyotypic relationships among Equus grevyi, Equus burchelli and domestic horse defined using horse chromosome arm-specific probes
skos:prefLabel
Karyotypové vztahy mezi Equus grevyi, Equus burchelli a koněm domácím definované pomocí sond specifických pro ramena koňských chromosomů Karyotypic relationships among Equus grevyi, Equus burchelli and domestic horse defined using horse chromosome arm-specific probes Karyotypic relationships among Equus grevyi, Equus burchelli and domestic horse defined using horse chromosome arm-specific probes
skos:notation
RIV/00027162:_____/07:#0000257!RIV08-MZE-00027162
n3:strany
807;813
n3:aktivita
n7:P n7:Z
n3:aktivity
P(GA523/06/1402), Z(MZE0002716201)
n3:cisloPeriodika
6
n3:dodaniDat
n14:2008
n3:domaciTvurceVysledku
n4:8164703 n4:8408033 n4:3191907
n3:druhVysledku
n17:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
428836
n3:idVysledku
RIV/00027162:_____/07:#0000257
n3:jazykVysledku
n9:eng
n3:klicovaSlova
Equus grevyi; laser microdissection; arm-specific probes; Equus caballus; Equus burchelli; comparative painting
n3:klicoveSlovo
n6:laser%20microdissection n6:Equus%20caballus n6:comparative%20painting n6:arm-specific%20probes n6:Equus%20burchelli n6:Equus%20grevyi
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[DB9300BA77C5]
n3:nazevZdroje
Chromosome Research
n3:obor
n13:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
6
n3:projekt
n8:GA523%2F06%2F1402
n3:rokUplatneniVysledku
n14:2007
n3:svazekPeriodika
15
n3:tvurceVysledku
Váhala, J. Hořín, P. Musilová, Petra Zrnová, E. Kubíčková, Svatava Rubeš, Jiří
n3:zamer
n16:MZE0002716201
s:issn
0967-3849
s:numberOfPages
7