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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F05%3A06G00208%21RIV06-GA0-00027162
rdf:type
n9:Vysledek skos:Concept
dcterms:description
Byla zjišťována přítomnost shigatoxigenních E. coli (STEC) v rektálních výtěrech jehňat a kůzlat ze dvou extenzivních a dvou intenzivních chovů v Jordánsku. V jednom chovu ovcí s intenzivní mléčnou produkcí byly STEC O157:H7 detekovány u 8 ze 32 jehňattrpících průjmem. V ostatních chovech převládaly nálezy STEC séroskupin O128, O78, O15 a O128:K85. U všech STEC O157:H7 byla zjištěna přítomnost genů stx2, ehlyA a eaeA, u dvou netypovatelných kmenů byly zjištěny geny stx2 a ehlyA. U ostatních ne-O157STEC byly zjištěny pouze geny stx1 a ehlyA. Dále byla detekována antibiotická rezistence u všech STEC. Při porovnání multiresistentních STEC O157:H7 RAPD byly zjištěny mezi kmeny rozdíly. Izoláty STEC resistentní vůči antimikrobiálním látkám bylyzjištěny jen v intenzivních chovech. Ninety-three rectal swabs of lambs and young goats from two extensively and two intensively managed herds in Jordan were examined for Shiga toxin- producing E. coli (STEC). The STEC O157:H7 strains were demonstrated in 8 of 32 diarrheic lambs 1 to 3weeks old in one sheep herd with intensive milk production. In remaining herds, serogroups O128, O78, O15 and O128:K85 of STEC strains were most frequent findings. The presence of stx2, ehlyA and eaeA genes in all STEC O157:H7 isolates was confirmed byPCR, in two untypable STEC isolates, stx2 and ehlyA genes. In other STEC non-O157 isolates, only stx1 and ehlyA genes were found. STEC O157:H7 were resistant against sulphonamides and chloramphenicol, five were also resistant against ampicilin andstreptomycin, one agaist co-trimoxazole. Compared the resistant STEC O157:H7 isolates, the multiresistant isolate had a different RAPD patern. STEC isolates resistant against antimicrobial agents were demonstrated only in intensive herds. Ninety-three rectal swabs of lambs and young goats from two extensively and two intensively managed herds in Jordan were examined for Shiga toxin- producing E. coli (STEC). The STEC O157:H7 strains were demonstrated in 8 of 32 diarrheic lambs 1 to 3weeks old in one sheep herd with intensive milk production. In remaining herds, serogroups O128, O78, O15 and O128:K85 of STEC strains were most frequent findings. The presence of stx2, ehlyA and eaeA genes in all STEC O157:H7 isolates was confirmed byPCR, in two untypable STEC isolates, stx2 and ehlyA genes. In other STEC non-O157 isolates, only stx1 and ehlyA genes were found. STEC O157:H7 were resistant against sulphonamides and chloramphenicol, five were also resistant against ampicilin andstreptomycin, one agaist co-trimoxazole. Compared the resistant STEC O157:H7 isolates, the multiresistant isolate had a different RAPD patern. STEC isolates resistant against antimicrobial agents were demonstrated only in intensive herds.
dcterms:title
Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats in Jordan with evidence of multiresistant serotype O157:H7 Izolace a charakterizace shigatoxigenních Escherichia coli z ovcí a koz v Jordánsku se zaměřením na multirezistentní sérotyp O157:H7 Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats in Jordan with evidence of multiresistant serotype O157:H7
skos:prefLabel
Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats in Jordan with evidence of multiresistant serotype O157:H7 Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats in Jordan with evidence of multiresistant serotype O157:H7 Izolace a charakterizace shigatoxigenních Escherichia coli z ovcí a koz v Jordánsku se zaměřením na multirezistentní sérotyp O157:H7
skos:notation
RIV/00027162:_____/05:06G00208!RIV06-GA0-00027162
n3:strany
111;118
n3:aktivita
n4:P
n3:aktivity
P(GA525/00/0666)
n3:cisloPeriodika
3
n3:dodaniDat
n12:2006
n3:domaciTvurceVysledku
n14:6757669
n3:druhVysledku
n15:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
525742
n3:idVysledku
RIV/00027162:_____/05:06G00208
n3:jazykVysledku
n8:eng
n3:klicovaSlova
lamb; goat; shiga toxin; stx1; stx2; eaeA; ehlyA; antibiotic resistance
n3:klicoveSlovo
n5:lamb n5:goat n5:ehlyA n5:stx1 n5:stx2 n5:antibiotic%20resistance n5:shiga%20toxin n5:eaeA
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[BAC39C8904AD]
n3:nazevZdroje
Veterinární medicína
n3:obor
n17:EE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
6
n3:projekt
n16:GA525%2F00%2F0666
n3:rokUplatneniVysledku
n12:2005
n3:svazekPeriodika
50
n3:tvurceVysledku
Alexa, Pavel
s:issn
0375-8427
s:numberOfPages
8