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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F05%3A06G00201%21RIV06-GA0-00027162
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Leukocyte influx induction was accomplished by using four inducers: i) sterile buffered saline solution (PBS); ii) 5% glucose solution (GLU); iii) synthetic muramyl dipeptide analogue (MDP); and iv) lipopolysaccharide (LPS), administered into themammary gland lumen. The total cell counts in lavages increased after treatment by all inducers in comparison to the counts before influx induction (P<0.001). Cell counts were higher and differed significantly by MDP and LPS (P<0.01) in contrast to PBS.The highest proportion of neutrophils was induced by LPS (P<0.01). After three-hour incubation, light microscopy examination revealed the highest manifestation of neutrophil apoptosis after induction by GLU (P<0.05). Determination of early manifestationof neutrophil apoptosis revealed the lowest manifestation of neutrophil apoptosis after LPS (P<0.01). The results of late manifestation of neutrophil apoptosis revealed the highest proportion of apoptotic neutrophils after induction by GLU (P<0.05). Leukocyte influx induction was accomplished by using four inducers: i) sterile buffered saline solution (PBS); ii) 5% glucose solution (GLU); iii) synthetic muramyl dipeptide analogue (MDP); and iv) lipopolysaccharide (LPS), administered into themammary gland lumen. The total cell counts in lavages increased after treatment by all inducers in comparison to the counts before influx induction (P<0.001). Cell counts were higher and differed significantly by MDP and LPS (P<0.01) in contrast to PBS.The highest proportion of neutrophils was induced by LPS (P<0.01). After three-hour incubation, light microscopy examination revealed the highest manifestation of neutrophil apoptosis after induction by GLU (P<0.05). Determination of early manifestationof neutrophil apoptosis revealed the lowest manifestation of neutrophil apoptosis after LPS (P<0.01). The results of late manifestation of neutrophil apoptosis revealed the highest proportion of apoptotic neutrophils after induction by GLU (P<0.05). Influx leukocytů byl indukován čtyřmi induktory: a) sterilním pufrovaným fyziologickým roztokem (PBS, b) 5% roztokem glukózy (GLU), c) syntetickým analogem muramyldipeptidu (MDP) a d) lipopolysacharidem (LPS), které byly aplikovány do lumina mléčnýchžláz. Celkový počet buněk v lavážích vzrostl po aplikaci všech induktorů ve srovnání s počtem před indukcí influxu (P<0.001). Vyšší a statisticky významně diferentní byl počet buněk po MDP a LPS (P<0.01) oproti PBS. Nejvyšší podíl neutrofilů bylindukován LPS (P<0.01). Po třech hodinách inkubace odchalila světelná mikroskopie nejvyšší manifestaci apoptózy neutrofilů po indukci GLU (P<0.05). Stanovení časných projevů apoptózy neutrofilů odhalilo nejnižší manifestaci po LPS (P<0.01). Výsledkypozdní manifestace apoptózy neutrofilů odhalily nejvyšší proporci apoptotických neutrofilů po indukci GLU (P<0.05).
dcterms:title
Effects of certain inducers of leukocytes migration into the bovine mammary gland on neutrophil apoptosis manifestation in a subsequent in vitro cultivation Účinek určitých indukátorů migrace leukocytů do mléčné žlázy skotu na manifestaci neutrofilové apoptózy při následní kultivaci in vitro Effects of certain inducers of leukocytes migration into the bovine mammary gland on neutrophil apoptosis manifestation in a subsequent in vitro cultivation
skos:prefLabel
Effects of certain inducers of leukocytes migration into the bovine mammary gland on neutrophil apoptosis manifestation in a subsequent in vitro cultivation Účinek určitých indukátorů migrace leukocytů do mléčné žlázy skotu na manifestaci neutrofilové apoptózy při následní kultivaci in vitro Effects of certain inducers of leukocytes migration into the bovine mammary gland on neutrophil apoptosis manifestation in a subsequent in vitro cultivation
skos:notation
RIV/00027162:_____/05:06G00201!RIV06-GA0-00027162
n3:strany
305;312
n3:aktivita
n11:P
n3:aktivity
P(GA524/01/1290)
n3:cisloPeriodika
3
n3:dodaniDat
n9:2006
n3:domaciTvurceVysledku
n7:8859027
n3:druhVysledku
n12:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
519565
n3:idVysledku
RIV/00027162:_____/05:06G00201
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Heifers; Virgin mammary gland; Leukocyte influx; Inducers; Neutrophils; Apoptosis
n3:klicoveSlovo
n4:Heifers n4:Leukocyte%20influx n4:Neutrophils n4:Virgin%20mammary%20gland n4:Inducers n4:Apoptosis
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[AE003F44953B]
n3:nazevZdroje
Physiological Research
n3:obor
n14:EC
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n13:GA524%2F01%2F1290
n3:rokUplatneniVysledku
n9:2005
n3:svazekPeriodika
54
n3:tvurceVysledku
Ryšánek, Dušan
s:issn
0862-8408
s:numberOfPages
8