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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F04%3A00012679%21RIV%2F2005%2FGA0%2FM03005%2FN
rdf:type
n11:Vysledek skos:Concept
dcterms:description
River buffalo, sheep and goat spermatozoa were cross-hybridized for double color FISH with bovine X and Y-chromosome painting probes, prepared by DOP-PCR of laser-microdissected-catapulted chromosomes, to investigate the possibility of using bovine probes for sexing sperm of other members of the family Bovidae. FISH analysis of more than twelve thousands sperm revealed high level of X-Y signals on all species investigated. Significant interspecific differences (P<0.01) were detected in the frequency of aberrant spermatozoa (aneuploid and diploid) between goat (0.393%) and sheep (0.033%) followed by river buffalo (0.224%) and cattle (0.096%). The present study demonstrated that it is possible to use bovine X-Y painting probes for sexing and analyzing sperm of other species of the family, thus facilitating future studies on the incidence of chromosome abnormalities in sperm as well as on sex predetermination of embryos for the livestock industry. River buffalo, sheep and goat spermatozoa were cross-hybridized for double color FISH with bovine X and Y-chromosome painting probes, prepared by DOP-PCR of laser-microdissected-catapulted chromosomes, to investigate the possibility of using bovine probes for sexing sperm of other members of the family Bovidae. FISH analysis of more than twelve thousands sperm revealed high level of X-Y signals on all species investigated. Significant interspecific differences (P<0.01) were detected in the frequency of aberrant spermatozoa (aneuploid and diploid) between goat (0.393%) and sheep (0.033%) followed by river buffalo (0.224%) and cattle (0.096%). The present study demonstrated that it is possible to use bovine X-Y painting probes for sexing and analyzing sperm of other species of the family, thus facilitating future studies on the incidence of chromosome abnormalities in sperm as well as on sex predetermination of embryos for the livestock industry. Spermie buvola vodního, ovce a kozy byly hybridizovány dvoubarevnou FISH s boviními malovacími sondami připravenými pomocí mikrodisekce a DOP-PCR. Signifikantní mezidruhové diference (P<0.01) byly zjištěny ve frekvenci aberantních spermií (aneuploidie a diploidie) mezi kozou (0.393%), ovcí (0.033%), buvolem vodním (0.224%) a skotem (0.096%). Bylo demonstrováno, že boviní malovací sondy pro chromozom X a Y lze použít pro sexování a analýzu spermií ostatních druhů čeledi Bovidae.
dcterms:title
Sexing river buffalo (Bubalus bubalis L.), sheep (Ovis aries L.), goat (Capra hircus L.), and cattle spermatozoa by double color FISH using X- and Y- bovine (Bos taurus L.) painting probes Sexování spermií búvola vodního (Bubalus bubalis L.), ovce (Ovis aries L.), kozy (Capra hircus L.) a skotu (Bos taurus L.) dvoubarevnou s použitím X a Y malovacích sond Sexing river buffalo (Bubalus bubalis L.), sheep (Ovis aries L.), goat (Capra hircus L.), and cattle spermatozoa by double color FISH using X- and Y- bovine (Bos taurus L.) painting probes
skos:prefLabel
Sexování spermií búvola vodního (Bubalus bubalis L.), ovce (Ovis aries L.), kozy (Capra hircus L.) a skotu (Bos taurus L.) dvoubarevnou s použitím X a Y malovacích sond Sexing river buffalo (Bubalus bubalis L.), sheep (Ovis aries L.), goat (Capra hircus L.), and cattle spermatozoa by double color FISH using X- and Y- bovine (Bos taurus L.) painting probes Sexing river buffalo (Bubalus bubalis L.), sheep (Ovis aries L.), goat (Capra hircus L.), and cattle spermatozoa by double color FISH using X- and Y- bovine (Bos taurus L.) painting probes
skos:notation
RIV/00027162:_____/04:00012679!RIV/2005/GA0/M03005/N
n3:strany
108;115
n3:aktivita
n15:P
n3:aktivity
P(GA523/01/0291)
n3:cisloPeriodika
1
n3:dodaniDat
n13:2005
n3:domaciTvurceVysledku
n4:8164703
n3:druhVysledku
n8:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n5:predkladatel
n3:idSjednocenehoVysledku
586035
n3:idVysledku
RIV/00027162:_____/04:00012679
n3:jazykVysledku
n10:eng
n3:klicovaSlova
sexing;spermatozoa;FISH;river buffalo;sheep;goat;cattle
n3:klicoveSlovo
n6:sheep n6:sexing n6:spermatozoa n6:cattle n6:FISH n6:river%20buffalo n6:goat
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[7FB98C40DE79]
n3:nazevZdroje
Molecular Reproduction and Development
n3:obor
n12:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
8
n3:projekt
n17:GA523%2F01%2F0291
n3:rokUplatneniVysledku
n13:2004
n3:svazekPeriodika
67
n3:tvurceVysledku
Jiří, Rubeš
s:issn
1040-452X
s:numberOfPages
8