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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F01%3A80000040%21RIV%2F2002%2FMZE%2FM03002%2FN
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Very few data on the incidence and risk factors for aneuploidy, as a significant cause of reproductive disorders in farm animals, are available. Most aneuploid conceptuses die in utero and methods for early assessnebt of aneuploidy are needed. Porcine embryos were collected, transferred onto poly-L lysine-coated slides and fixed with 0.01 N HCl, 0.1 % Tween 20. A method of multi-color fluorescence in situ hybridisation was developed for detecting chromosomes 1, 10 and Y in blastomeres. Only embryos with extra signals were regarded as pathological. Eighty-five of the 93 examined embryos (91.4%) were diploid, 2 (2.6%) were tripolid, 5 (5.4%) were mosaic, 1 (1.1%) showed trisomy, and 44 (47.3%) showed 1 chromosome Y signal. Very few data on the incidence and risk factors for aneuploidy, as a significant cause of reproductive disorders in farm animals, are available. Most aneuploid conceptuses die in utero and methods for early assessnebt of aneuploidy are needed. Porcine embryos were collected, transferred onto poly-L lysine-coated slides and fixed with 0.01 N HCl, 0.1 % Tween 20. A method of multi-color fluorescence in situ hybridisation was developed for detecting chromosomes 1, 10 and Y in blastomeres. Only embryos with extra signals were regarded as pathological. Eighty-five of the 93 examined embryos (91.4%) were diploid, 2 (2.6%) were tripolid, 5 (5.4%) were mosaic, 1 (1.1%) showed trisomy, and 44 (47.3%) showed 1 chromosome Y signal.
dcterms:title
Aneuploidy detection in porcine embryos using fluorescence in situ hybridization Aneuploidy detection in porcine embryos using fluorescence in situ hybridization
skos:prefLabel
Aneuploidy detection in porcine embryos using fluorescence in situ hybridization Aneuploidy detection in porcine embryos using fluorescence in situ hybridization
skos:notation
RIV/00027162:_____/01:80000040!RIV/2002/MZE/M03002/N
n3:strany
77;78
n3:aktivita
n15:Z
n3:aktivity
Z(MZE-M03-99-01)
n3:cisloPeriodika
suppl.1
n3:dodaniDat
n6:2002
n3:domaciTvurceVysledku
n14:9403205
n3:druhVysledku
n4:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
673394
n3:idVysledku
RIV/00027162:_____/01:80000040
n3:jazykVysledku
n11:eng
n3:klicovaSlova
Aneuploidy, porcine embryos, fluorescence in situ hybridisation.
n3:klicoveSlovo
n5:Aneuploidy n5:fluorescence%20in%20situ%20hybridisation. n5:porcine%20embryos
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[EBCB9B859795]
n3:nazevZdroje
Chromosome research (14th International Chromosome Conference)
n3:obor
n7:EB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:rokUplatneniVysledku
n6:2001
n3:svazekPeriodika
9
n3:tvurceVysledku
Řezáčová, Olga
n3:zamer
n13:MZE-M03-99-01
s:issn
0967-3849
s:numberOfPages
2