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Statements

Subject Item
n2:RIV%2F00027073%3A_____%2F13%3A%230001614%21RIV14-MZE-00027073
rdf:type
n3:Vysledek skos:Concept
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0304423813003294
dcterms:description
A protocol is described for the adventitious shoot bud regeneration from leaf and stem explants in A. hippocastanum. Effect of different media (Heller, WPM, Rugini and DKW) and growth regulators (BA, TDZ and NAA) combinations on shoot organogenesis were examined. The highest shoot number and length was achieved on Rugini and WPM medium. Adventitious shoot formation was observed in all types of explants. Regeneration frequency and shoot number depended on explant type and growth regulators. The highest regeneration (100%) and the highest shoot bud number (9.7) were recorded in stem explants on WPM medium with 8.9 µM BA + 0.5 µM NAA. Generally, TDZ was less effective on shoot regeneration in stem and leaf explants compared to BA. Rooting of elongated shoots was significantly increased (68.1%) on full-strength WPM medium supplemented with 2.7 µM NAA. Rooted plantlets were acclimatized successfully under greenhouse conditions. A protocol is described for the adventitious shoot bud regeneration from leaf and stem explants in A. hippocastanum. Effect of different media (Heller, WPM, Rugini and DKW) and growth regulators (BA, TDZ and NAA) combinations on shoot organogenesis were examined. The highest shoot number and length was achieved on Rugini and WPM medium. Adventitious shoot formation was observed in all types of explants. Regeneration frequency and shoot number depended on explant type and growth regulators. The highest regeneration (100%) and the highest shoot bud number (9.7) were recorded in stem explants on WPM medium with 8.9 µM BA + 0.5 µM NAA. Generally, TDZ was less effective on shoot regeneration in stem and leaf explants compared to BA. Rooting of elongated shoots was significantly increased (68.1%) on full-strength WPM medium supplemented with 2.7 µM NAA. Rooted plantlets were acclimatized successfully under greenhouse conditions.
dcterms:title
Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.) Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.)
skos:prefLabel
Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.) Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.)
skos:notation
RIV/00027073:_____/13:#0001614!RIV14-MZE-00027073
n3:predkladatel
n13:ico%3A00027073
n4:aktivita
n15:P
n4:aktivity
P(QH81101)
n4:cisloPeriodika
24 September 2013
n4:dodaniDat
n7:2014
n4:domaciTvurceVysledku
n9:5200423 n9:6295932
n4:druhVysledku
n17:J
n4:duvernostUdaju
n8:S
n4:entitaPredkladatele
n10:predkladatel
n4:idSjednocenehoVysledku
104962
n4:idVysledku
RIV/00027073:_____/13:#0001614
n4:jazykVysledku
n16:eng
n4:klicovaSlova
Rooting; Adventitious shoot regeneration; Stem explant; in vitro; Leaf explant
n4:klicoveSlovo
n6:Rooting n6:Leaf%20explant n6:Adventitious%20shoot%20regeneration n6:Stem%20explant n6:in%20vitro
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[025581AABE8F]
n4:nazevZdroje
Scientia Horticulturae
n4:obor
n11:EI
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
3
n4:projekt
n19:QH81101
n4:rokUplatneniVysledku
n7:2013
n4:svazekPeriodika
161
n4:tvurceVysledku
Mertelík, Josef Šedivá, Jana Vlašínová, Helena
n4:wos
000325595300033
s:issn
0304-4238
s:numberOfPages
5
n20:doi
10.1016/j.scienta.2013.06.030