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Statements

Subject Item
n2:RIV%2F00027073%3A_____%2F10%3A%230000838%21RIV11-MZP-00027073
rdf:type
n7:Vysledek skos:Concept
dcterms:description
The objective of the work was to apply selected genetic markers to verify the genetic purity of bog pine (Pinus uncinata subsp. uliginosa) individuals from the Borkovická blata population in order to initiate in vitro cultures from them. The plastid DNA analysis was used as a molecular-genetic tool. The trnL-trnF region of chloroplast DNA was tested by PCR-RFLP method; furthermore, the chloroplast microsatellite region Pt41093 was also analysed. All the tested individuals from the studied population matched the Pinus uncinata subsp. uliginosa haplotype. In bog pine, regeneration and development of axillary shoots were significantly enhanced in the presence of 5 g/L activated charcoal. The objective of the work was to apply selected genetic markers to verify the genetic purity of bog pine (Pinus uncinata subsp. uliginosa) individuals from the Borkovická blata population in order to initiate in vitro cultures from them. The plastid DNA analysis was used as a molecular-genetic tool. The trnL-trnF region of chloroplast DNA was tested by PCR-RFLP method; furthermore, the chloroplast microsatellite region Pt41093 was also analysed. All the tested individuals from the studied population matched the Pinus uncinata subsp. uliginosa haplotype. In bog pine, regeneration and development of axillary shoots were significantly enhanced in the presence of 5 g/L activated charcoal.
dcterms:title
Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa) Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa)
skos:prefLabel
Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa) Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa)
skos:notation
RIV/00027073:_____/10:#0000838!RIV11-MZP-00027073
n4:aktivita
n16:P
n4:aktivity
P(SP/2D4/83/07)
n4:cisloPeriodika
8
n4:dodaniDat
n15:2011
n4:domaciTvurceVysledku
n10:4662369 n10:6471633
n4:druhVysledku
n17:J
n4:duvernostUdaju
n12:S
n4:entitaPredkladatele
n9:predkladatel
n4:idSjednocenehoVysledku
287201
n4:idVysledku
RIV/00027073:_____/10:#0000838
n4:jazykVysledku
n5:eng
n4:klicovaSlova
DNA extraction; in vitro regeneration; plastid DNA; seedling shoot apices
n4:klicoveSlovo
n13:seedling%20shoot%20apices n13:plastid%20DNA n13:DNA%20extraction n13:in%20vitro%20regeneration
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[31CB0E2A7FCF]
n4:nazevZdroje
Journal of Forest Science
n4:obor
n11:GK
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
2
n4:projekt
n14:SP%2F2D4%2F83%2F07
n4:rokUplatneniVysledku
n15:2010
n4:svazekPeriodika
56
n4:tvurceVysledku
Vejsadová, Hana Lukášová, Miroslava
s:issn
1212-4834
s:numberOfPages
7