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Statements

Subject Item
n2:RIV%2F00027022%3A_____%2F13%3A00000378%21RIV14-MZE-00027022
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1080/08957959.2013.828716
dcterms:description
Polyphenol oxidase (PPO) has been shown to reduce major peanut allergens. Since high pressure (HP) can increase enzyme activity, we postulated that further reduction of peanut allergens can be achieved through HP combined with PPO. Peanut extracts containing caffeic acid were treated with each of the following: (1) HP; (2) HP + PPO; (3) PPO; and (4) none. HP was conducted at 300 and 500MPa, each for 3 and 10 min, 37 °C. After treatment, SDS-PAGE was performed and allergenic capacity (IgE binding) was determined colorimetrically in inhibition enzyme-linked immunosorbent assay and Western blots, using a pooled plasma from peanut-allergic patients. Data showed that HP alone had no effect on major peanut allergens. However, HP at 500MPa combined with PPO (HP500/PPO) induced a higher (approximately twofold) reduction of major peanut allergens and IgE binding than PPO alone or HP300/PPO. There was no difference between treatment times. We concluded that HP500/PPO at 3-min enhanced a twofold reduction of the allergenic capacity of peanut extracts, as compared to PPO itself. Polyphenol oxidase (PPO) has been shown to reduce major peanut allergens. Since high pressure (HP) can increase enzyme activity, we postulated that further reduction of peanut allergens can be achieved through HP combined with PPO. Peanut extracts containing caffeic acid were treated with each of the following: (1) HP; (2) HP + PPO; (3) PPO; and (4) none. HP was conducted at 300 and 500MPa, each for 3 and 10 min, 37 °C. After treatment, SDS-PAGE was performed and allergenic capacity (IgE binding) was determined colorimetrically in inhibition enzyme-linked immunosorbent assay and Western blots, using a pooled plasma from peanut-allergic patients. Data showed that HP alone had no effect on major peanut allergens. However, HP at 500MPa combined with PPO (HP500/PPO) induced a higher (approximately twofold) reduction of major peanut allergens and IgE binding than PPO alone or HP300/PPO. There was no difference between treatment times. We concluded that HP500/PPO at 3-min enhanced a twofold reduction of the allergenic capacity of peanut extracts, as compared to PPO itself.
dcterms:title
Reducing Peanut Allergens by High Pressure Combined with Polyphenol Oxidase Reducing Peanut Allergens by High Pressure Combined with Polyphenol Oxidase
skos:prefLabel
Reducing Peanut Allergens by High Pressure Combined with Polyphenol Oxidase Reducing Peanut Allergens by High Pressure Combined with Polyphenol Oxidase
skos:notation
RIV/00027022:_____/13:00000378!RIV14-MZE-00027022
n5:predkladatel
n14:ico%3A00027022
n3:aktivita
n4:Z
n3:aktivity
Z(MZE0002702202)
n3:cisloPeriodika
4
n3:dodaniDat
n8:2014
n3:domaciTvurceVysledku
n19:2102366
n3:druhVysledku
n16:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
101840
n3:idVysledku
RIV/00027022:_____/13:00000378
n3:jazykVysledku
n15:eng
n3:klicovaSlova
high pressure; polyphenol oxidase; PPO; peanut allergens; Ara h 1 and Ara h 2; IgE antibodies
n3:klicoveSlovo
n10:IgE%20antibodies n10:PPO n10:peanut%20allergens n10:Ara%20h%201%20and%20Ara%20h%202 n10:high%20pressure n10:polyphenol%20oxidase
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[4126535AF92E]
n3:nazevZdroje
High Pressure Research,print
n3:obor
n11:GM
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n8:2013
n3:svazekPeriodika
33
n3:tvurceVysledku
Reed, Shawndrika Chung, Si-Yin Houška, Milan
n3:wos
000327931000012
n3:zamer
n18:MZE0002702202
s:issn
0895-7959
s:numberOfPages
9