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Statements

Subject Item
n2:RIV%2F00027022%3A_____%2F11%3A00000233%21RIV12-MZE-00027022
rdf:type
n10:Vysledek skos:Concept
dcterms:description
A collaborative study in 12 laboratories was performed to prove the validation of the ELISA method developed for the quantitative mustard protein determination in foods. The ELISA kit used for this study is based on rabbit polyclonal antibody. This kit did not produce any false positive results or cross-reactivity in in-house validation with a broad range of food matrices range with no detectable mustard protein . All participants obtained the Mustard ELISA kit with standard operational procedures, the list of samples, samples and a protocol for recording test results. The study included 15 food samples and 2 spiked samples. The statistical tests (Cochran, Dixon and Mandel) and analysis of variance (ANOVA) were used for the evaluation of the collaborative study results. Repeatability and reproducibility limits as well as a limit of quantification (LOQ, 1.8 mg mustard proteins/kg) and a limit of detection (LOD, 0.5 mg mustard proteins/kg) for the kit were calculated. A collaborative study in 12 laboratories was performed to prove the validation of the ELISA method developed for the quantitative mustard protein determination in foods. The ELISA kit used for this study is based on rabbit polyclonal antibody. This kit did not produce any false positive results or cross-reactivity in in-house validation with a broad range of food matrices range with no detectable mustard protein . All participants obtained the Mustard ELISA kit with standard operational procedures, the list of samples, samples and a protocol for recording test results. The study included 15 food samples and 2 spiked samples. The statistical tests (Cochran, Dixon and Mandel) and analysis of variance (ANOVA) were used for the evaluation of the collaborative study results. Repeatability and reproducibility limits as well as a limit of quantification (LOQ, 1.8 mg mustard proteins/kg) and a limit of detection (LOD, 0.5 mg mustard proteins/kg) for the kit were calculated.
dcterms:title
ELISA Kit for Mustard Protein Determination: Interlaboratory study ELISA Kit for Mustard Protein Determination: Interlaboratory study
skos:prefLabel
ELISA Kit for Mustard Protein Determination: Interlaboratory study ELISA Kit for Mustard Protein Determination: Interlaboratory study
skos:notation
RIV/00027022:_____/11:00000233!RIV12-MZE-00027022
n10:predkladatel
n11:ico%3A00027022
n3:aktivita
n6:Z
n3:aktivity
Z(MZE0002702202)
n3:cisloPeriodika
2
n3:dodaniDat
n14:2012
n3:domaciTvurceVysledku
n4:4379950 n4:8900426 n4:5221900
n3:druhVysledku
n15:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
197277
n3:idVysledku
RIV/00027022:_____/11:00000233
n3:jazykVysledku
n13:eng
n3:klicovaSlova
ELISA method; determination; mustard proteins
n3:klicoveSlovo
n9:ELISA%20method n9:mustard%20proteins n9:determination
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[A613C0F943E7]
n3:nazevZdroje
Journal of AOAC International
n3:obor
n18:GM
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
27
n3:rokUplatneniVysledku
n14:2011
n3:svazekPeriodika
94
n3:tvurceVysledku
Jimenez, Esther Takkinen, Kristina Tomková, Květa Malmheden Yman, Ingrid Piknová, Lubica Baranda, Ana Martinez de Maranon, Inigo Postigo Reza, Idoia Kubík, Martin Štumr, František Guisantes Del Barco, Jorge Antonio Cuhra, Petr Martinez Quesada, Jorge Bulawová, Hana Rysová, Jana Brychta, Josef Baršová, Soňa Dvorská, Pavla Iametti, Stefania Laukkanen, Marja-Leena Gabrovská, Dana Pardo, Miguel Angel Čenčič, Avrelija Hanák, Petr Plicka, Jan Sunen Pardo, Esther Langerholc, Tomaz
n3:wos
000289544500029
n3:zamer
n8:MZE0002702202
s:issn
1060-3271
s:numberOfPages
6