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Statements

Subject Item
n2:RIV%2F00027022%3A_____%2F09%3A00000129%21RIV10-MSM-00027022
rdf:type
skos:Concept n12:Vysledek
dcterms:description
Cyclodextrins (CDs) are synthesized by the bacterial extracellular enzym cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) from starch or starch derivatives via an inter- and intramolecural transglycosylation reaction. The production of ?- CD, ß- CD and ?- CD by CGTase from Paenibacillus macerans was studied, in regard to the effect of the source starch (amaranth, corn) on the yield of CDs. The CGTase was produced by a 3-day sterile cultivation in the laboratory Bench-top fermentor BiostatB in a complex medium containing starch and partially purified by ammonium sulphate precipitation at 60% saturation. Apparent molecular weight as determined by SDS–PAGE was in the range from 105 to 114 kDa. All the commercially important ?-, ß-, and ?-CDs were detected chromatographically after hydrolysis of the corn and amaranth (Amaranthus cruentus) starch with the isolated enzyme. Cyclodextrins (CDs) are synthesized by the bacterial extracellular enzym cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) from starch or starch derivatives via an inter- and intramolecural transglycosylation reaction. The production of ?- CD, ß- CD and ?- CD by CGTase from Paenibacillus macerans was studied, in regard to the effect of the source starch (amaranth, corn) on the yield of CDs. The CGTase was produced by a 3-day sterile cultivation in the laboratory Bench-top fermentor BiostatB in a complex medium containing starch and partially purified by ammonium sulphate precipitation at 60% saturation. Apparent molecular weight as determined by SDS–PAGE was in the range from 105 to 114 kDa. All the commercially important ?-, ß-, and ?-CDs were detected chromatographically after hydrolysis of the corn and amaranth (Amaranthus cruentus) starch with the isolated enzyme.
dcterms:title
Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea) Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea)
skos:prefLabel
Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea) Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea)
skos:notation
RIV/00027022:_____/09:00000129!RIV10-MSM-00027022
n3:aktivita
n10:P
n3:aktivity
P(2B06173)
n3:dodaniDat
n4:2010
n3:domaciTvurceVysledku
n9:1955071 n9:1428918 n9:2806762 n9:4765419 n9:3881261
n3:druhVysledku
n11:O
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
331971
n3:idVysledku
RIV/00027022:_____/09:00000129
n3:jazykVysledku
n13:eng
n3:klicovaSlova
Cyclodextrin; fermentation; starch
n3:klicoveSlovo
n8:Cyclodextrin n8:fermentation n8:starch
n3:kontrolniKodProRIV
[6F0BFD26BCA4]
n3:obor
n15:CE
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n14:2B06173
n3:rokUplatneniVysledku
n4:2009
n3:tvurceVysledku
Molík, Petr Drahorád, Josef Urban, Marian Matušová, Krystina Adámek, Lubomír Beran, Miloš