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Statements

Subject Item
n2:RIV%2F00027022%3A_____%2F05%3A8P012110%21RIV08-MZE-00027022
rdf:type
n3:Vysledek skos:Concept
rdfs:seeAlso
http://www.vupp.cz/czvupp/publik/05poster/05FloridaPoster2005.pdf
dcterms:description
Using RP HPLC with methanol - water and acetonitril - water elution gradients and Gel Permeation HPLC the major proteins (?-lactalbumin, ß-lactoglobulin, serumalbumin) of bovine whey, except immunoglobulins, were identified and quantified by external standards method. Sandwich ELISAs with antibodies against ?-lactalbumin, ß-lactoglobulin, and serumalbumin were applied in parallel for quantitative analysis of selected protein fractions from the chromatographic separations. Results of the chromatographic and immunochemical quantification are compared and discussed. Porovnání výsledků stanovení hlavních bílkovin kravské syrovátky metodou HPLC a sendvičové ELISY. Using RP HPLC with methanol - water and acetonitril - water elution gradients and Gel Permeation HPLC the major proteins (?-lactalbumin, ß-lactoglobulin, serumalbumin) of bovine whey, except immunoglobulins, were identified and quantified by external standards method. Sandwich ELISAs with antibodies against ?-lactalbumin, ß-lactoglobulin, and serumalbumin were applied in parallel for quantitative analysis of selected protein fractions from the chromatographic separations. Results of the chromatographic and immunochemical quantification are compared and discussed.
dcterms:title
POSTER: Beran, M., Hanak, P., Molik, P., Urban, M., and Zamecnikova, I.: Simultaneous identification and quantification of major bovine whey proteins by HPLC and Sandwich ELISAs. POSTER: Beran, M., Hanak, P., Molik, P., Urban, M., and Zamecnikova, I.: Simultaneous identification and quantification of major bovine whey proteins by HPLC and Sandwich ELISAs. POSTER: Beran, M., Hanak, P., Molik, P., Urban, M., and Zamecnikova, I.: Souběžná identifikace a kvantitativní stanovení hlavních bílkovin kravské syrovátky metodani HPLC a sendvičová ELISA.
skos:prefLabel
POSTER: Beran, M., Hanak, P., Molik, P., Urban, M., and Zamecnikova, I.: Simultaneous identification and quantification of major bovine whey proteins by HPLC and Sandwich ELISAs. POSTER: Beran, M., Hanak, P., Molik, P., Urban, M., and Zamecnikova, I.: Souběžná identifikace a kvantitativní stanovení hlavních bílkovin kravské syrovátky metodani HPLC a sendvičová ELISA. POSTER: Beran, M., Hanak, P., Molik, P., Urban, M., and Zamecnikova, I.: Simultaneous identification and quantification of major bovine whey proteins by HPLC and Sandwich ELISAs.
skos:notation
RIV/00027022:_____/05:8P012110!RIV08-MZE-00027022
n4:aktivita
n18:P
n4:aktivity
P(QF3285)
n4:dodaniDat
n10:2008
n4:domaciTvurceVysledku
n7:1428918 n7:2806762 n7:1955071 n7:4379950 n7:6648460
n4:druhVysledku
n12:A
n4:duvernostUdaju
n15:S
n4:entitaPredkladatele
n13:predkladatel
n4:idSjednocenehoVysledku
537045
n4:idVysledku
RIV/00027022:_____/05:8P012110
n4:jazykVysledku
n11:eng
n4:klicovaSlova
whey proteins; HPLC; sandwich ELISA
n4:klicoveSlovo
n5:whey%20proteins n5:HPLC n5:sandwich%20ELISA
n4:kodPristupu
n17:V
n4:kontrolniKodProRIV
[C8CAADD15809]
n4:obor
n9:CE
n4:pocetDomacichTvurcuVysledku
5
n4:pocetTvurcuVysledku
5
n4:projekt
n14:QF3285
n4:rokUplatneniVysledku
n10:2005
n4:tvurceVysledku
Beran, Miloš Hanák, Petr Zámečníková, Ivana Urban, Marián Molík, Petr