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Statements

Subject Item
n2:RIV%2F00027014%3A_____%2F13%3A%230001922%21RIV14-MZE-00027014
rdf:type
skos:Concept n14:Vysledek
rdfs:seeAlso
http://www.agriculturejournals.cz/publicFiles/106669.pdf
dcterms:description
Oocyte ageing is a complex of processes that occur when matured in vitro oocytes are, after reaching the metaphase II stage, exposed to further in vitro culture. Aged oocytes remaining at the metaphase II stage undergo spontaneous parthenogenetic activation, or cellular death, through apoptosis (fragmentation) or lysis. The key factor in apoptotic pathway regulation is c-Jun-N-terminal kinase (JNK), stress kinase from the mitogene-activated protein kinase (MAPK) family. To investigate the effect of JNK inhibition on porcine oocytes ageing, cleavage rate, and embryonic development after parthenogenetic activation, DNA fragmentation, and pro-apoptotic factor Bax expression, we cultured in vitro matured oocytes for another 1–4 days in the presence of a JNK inhibitor. The inhibition of JNK significantly protected the oocytes from fragmentation (0% of fragmented oocytes under JNK inhibition vs. 13,4% of fragmented oocytes in the control group, 2nd day of ageing) and increased the percentage of parthenogenetically activated oocytes (82 vs 57,7%, 2nd day of ageing). The embryonic development of oocytes parthenogenetically activated after 24 h of ageing was influenced by JNK inhibition as well. The percentage of oocytes at the morula stage, after seven days of cultivation, was significantly increased when oocytes aged in the presence of a JNK inhibitor (42,5%) by comparison to the percentage of oocytes exposed to ageing in an inhibitor-free medium (23,3%). DNA fragmentation was significantly suppressed by JNK inhibition from the 1st day of ageing, but the expression of pro-apoptotic factor Bax in the oocytes was not influenced. On the basis of our experiments, we can conclude that JNK inhibition suppresses apoptosis and DNA fragmentation of aged oocytes and improves their embryonic development following the parthenogenetic activation. However, to completely eliminate all ageing related processes is insufficient. Oocyte ageing is a complex of processes that occur when matured in vitro oocytes are, after reaching the metaphase II stage, exposed to further in vitro culture. Aged oocytes remaining at the metaphase II stage undergo spontaneous parthenogenetic activation, or cellular death, through apoptosis (fragmentation) or lysis. The key factor in apoptotic pathway regulation is c-Jun-N-terminal kinase (JNK), stress kinase from the mitogene-activated protein kinase (MAPK) family. To investigate the effect of JNK inhibition on porcine oocytes ageing, cleavage rate, and embryonic development after parthenogenetic activation, DNA fragmentation, and pro-apoptotic factor Bax expression, we cultured in vitro matured oocytes for another 1–4 days in the presence of a JNK inhibitor. The inhibition of JNK significantly protected the oocytes from fragmentation (0% of fragmented oocytes under JNK inhibition vs. 13,4% of fragmented oocytes in the control group, 2nd day of ageing) and increased the percentage of parthenogenetically activated oocytes (82 vs 57,7%, 2nd day of ageing). The embryonic development of oocytes parthenogenetically activated after 24 h of ageing was influenced by JNK inhibition as well. The percentage of oocytes at the morula stage, after seven days of cultivation, was significantly increased when oocytes aged in the presence of a JNK inhibitor (42,5%) by comparison to the percentage of oocytes exposed to ageing in an inhibitor-free medium (23,3%). DNA fragmentation was significantly suppressed by JNK inhibition from the 1st day of ageing, but the expression of pro-apoptotic factor Bax in the oocytes was not influenced. On the basis of our experiments, we can conclude that JNK inhibition suppresses apoptosis and DNA fragmentation of aged oocytes and improves their embryonic development following the parthenogenetic activation. However, to completely eliminate all ageing related processes is insufficient.
dcterms:title
Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro
skos:prefLabel
Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro
skos:notation
RIV/00027014:_____/13:#0001922!RIV14-MZE-00027014
n14:predkladatel
n16:ico%3A00027014
n3:aktivita
n11:Z n11:I
n3:aktivity
I, Z(MZE0002701404)
n3:cisloPeriodika
12
n3:dodaniDat
n8:2014
n3:domaciTvurceVysledku
n12:9755330
n3:druhVysledku
n15:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
80208
n3:idVysledku
RIV/00027014:_____/13:#0001922
n3:jazykVysledku
n18:eng
n3:klicovaSlova
MAPK, DNA fragmentation, apoptosis, Bax
n3:klicoveSlovo
n4:Bax n4:MAPK n4:DNA%20fragmentation n4:apoptosis
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[6124FB8D1D16]
n3:nazevZdroje
Czech Journal of Animal Science
n3:obor
n5:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
8
n3:rokUplatneniVysledku
n8:2013
n3:svazekPeriodika
58
n3:tvurceVysledku
Nevoral, Jan Novotná, B. Sedmíková, Markéta Petr, Jaroslav Chmelíková, Eva Krejčová, Tereza Tůmová, Lenka Dörflerová, Andrea
n3:wos
000328563000001
n3:zamer
n19:MZE0002701404
s:issn
1212-1819
s:numberOfPages
11