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Statements

Subject Item
n2:RIV%2F00027014%3A_____%2F12%3A%230001661%21RIV13-MZE-00027014
rdf:type
n9:Vysledek skos:Concept
rdfs:seeAlso
http://www.vuzv.cz/sites/File/_privat/12104.pdf
dcterms:description
The aim of the present work was to determine the relative transcript abundance of the oestrogen receptor alpha (ER alpha) and beta (ER beta) gene in long-term cultures (up to 18 days) of pig granulosa cells (GC) obtained from small (1-2 mm; SF-GC) and large (5-7 mm; LF-GC) follicles. GC were cultured in the presence of 10% foetal calf serum (FCS) and one of the following growth factors: leukaemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF), or stem cell factor (SCF). The relative transcript abundance of the investigated genes was measured by real time PCR (RT-PCR). In comparison with the control (medium only), LIF significantly increased (P<0.05-0.01) ER alpha gene expression in SF-GC after 3, 15 and 18 days of culture and after 12 days in LF-GC. The ER beta mRNA level in SF-GC was significantly decreased (P<0.01) by all of the investigated factors after 3, 6, 9 and 12 days of incubation. In conclusion, expression of both types of oestrogen receptors during prolonged culture of pig GC under different conditions suggests the role of oestrogens acting via their receptors in maintaining survivability of porcine granulosa cells in vitro. The aim of the present work was to determine the relative transcript abundance of the oestrogen receptor alpha (ER alpha) and beta (ER beta) gene in long-term cultures (up to 18 days) of pig granulosa cells (GC) obtained from small (1-2 mm; SF-GC) and large (5-7 mm; LF-GC) follicles. GC were cultured in the presence of 10% foetal calf serum (FCS) and one of the following growth factors: leukaemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF), or stem cell factor (SCF). The relative transcript abundance of the investigated genes was measured by real time PCR (RT-PCR). In comparison with the control (medium only), LIF significantly increased (P<0.05-0.01) ER alpha gene expression in SF-GC after 3, 15 and 18 days of culture and after 12 days in LF-GC. The ER beta mRNA level in SF-GC was significantly decreased (P<0.01) by all of the investigated factors after 3, 6, 9 and 12 days of incubation. In conclusion, expression of both types of oestrogen receptors during prolonged culture of pig GC under different conditions suggests the role of oestrogens acting via their receptors in maintaining survivability of porcine granulosa cells in vitro.
dcterms:title
Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells
skos:prefLabel
Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells
skos:notation
RIV/00027014:_____/12:#0001661!RIV13-MZE-00027014
n9:predkladatel
n19:ico%3A00027014
n3:aktivita
n12:Z
n3:aktivity
Z(MZE0002701401)
n3:cisloPeriodika
2
n3:dodaniDat
n13:2013
n3:domaciTvurceVysledku
n15:4586395 n15:9470638
n3:druhVysledku
n6:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n5:predkladatel
n3:idSjednocenehoVysledku
163798
n3:idVysledku
RIV/00027014:_____/12:#0001661
n3:jazykVysledku
n14:eng
n3:klicovaSlova
pig, granulosa cells, oestrogen receptor, mRNA, prolonged culture, growth factors
n3:klicoveSlovo
n4:oestrogen%20receptor n4:growth%20factors n4:prolonged%20culture n4:mRNA n4:pig n4:granulosa%20cells
n3:kodStatuVydavatele
PL - Polská republika
n3:kontrolniKodProRIV
[BCE8D1B92E97]
n3:nazevZdroje
Journal of Animal and Feed Sciences
n3:obor
n17:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:rokUplatneniVysledku
n13:2012
n3:svazekPeriodika
21
n3:tvurceVysledku
Chronowska, Ewa Kott, Tomáš
n3:wos
000306726400008
n3:zamer
n11:MZE0002701401
s:issn
1230-1388
s:numberOfPages
11