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Statements

Subject Item
n2:RIV%2F00027014%3A_____%2F12%3A%230001634%21RIV13-MZE-00027014
rdf:type
skos:Concept n5:Vysledek
rdfs:seeAlso
http://www.vuzv.cz/sites/File/_privat/12075.pdf
dcterms:description
Genetic polymorphisms of bovine κ-casein (CSN3) and β-lactoglobulin (LGB) have been shown to affect milk compositional traits and technological properties. Monitoring individual CSN3 and LGB genetic variants in the population is important in the subsequent process of breeding cattle and improving milk protein quality. The objective of this study was to develop and validate a method for genotyping the main mutations of the bovine CSN3 and LGB genes using real time PCR (RT-PCR) followed by a high-resolution melting method (HRM) assay. The HRM-based assay allowed the sensitive and fast identification of the CSN3 AA, AB, BB, AE and BE genotypes and LGB AA, AB and BB genotypes simultaneously. This method was validated by analyzing 100 Fleckvieh cattle DNA samples, which were also genotyped by conventional methods at the molecular level using PCR–RFLP and DNA sequencing techniques. The genotypes obtained using the HRM approach perfectly matched the genotypes obtained by conventional analyses. Moreover, it is a cheap and fast alternative for existing methods. Genetic polymorphisms of bovine κ-casein (CSN3) and β-lactoglobulin (LGB) have been shown to affect milk compositional traits and technological properties. Monitoring individual CSN3 and LGB genetic variants in the population is important in the subsequent process of breeding cattle and improving milk protein quality. The objective of this study was to develop and validate a method for genotyping the main mutations of the bovine CSN3 and LGB genes using real time PCR (RT-PCR) followed by a high-resolution melting method (HRM) assay. The HRM-based assay allowed the sensitive and fast identification of the CSN3 AA, AB, BB, AE and BE genotypes and LGB AA, AB and BB genotypes simultaneously. This method was validated by analyzing 100 Fleckvieh cattle DNA samples, which were also genotyped by conventional methods at the molecular level using PCR–RFLP and DNA sequencing techniques. The genotypes obtained using the HRM approach perfectly matched the genotypes obtained by conventional analyses. Moreover, it is a cheap and fast alternative for existing methods.
dcterms:title
Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis
skos:prefLabel
Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis
skos:notation
RIV/00027014:_____/12:#0001634!RIV13-MZE-00027014
n5:predkladatel
n6:ico%3A00027014
n3:aktivita
n15:Z
n3:aktivity
Z(MZE0002701404)
n3:cisloPeriodika
1-3
n3:dodaniDat
n9:2013
n3:domaciTvurceVysledku
n11:2355000 n11:9281851 n11:7749252 n11:8284245
n3:druhVysledku
n17:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
168110
n3:idVysledku
RIV/00027014:_____/12:#0001634
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Cattle, CSN3, LGB, Genotyping, High-resolution melting
n3:klicoveSlovo
n7:CSN3 n7:Cattle n7:Genotyping n7:LGB n7:High-resolution%20melting
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[29AAF7F5AEF9]
n3:nazevZdroje
Livestock Science
n3:obor
n16:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n9:2012
n3:svazekPeriodika
145
n3:tvurceVysledku
Sztankóová, Zuzana Rychtářová, Jana Czerneková, Vladimíra Kyselová, Jitka
n3:wos
000303270400037
n3:zamer
n18:MZE0002701404
s:issn
1871-1413
s:numberOfPages
5
n13:doi
10.1016/j.livsci.2011.12.018