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Statements

Subject Item
n2:RIV%2F00027014%3A_____%2F11%3A%230001491%21RIV12-MZE-00027014
rdf:type
n15:Vysledek skos:Concept
rdfs:seeAlso
http://www.vuzv.cz/sites/File/_privat/11128.pdf
dcterms:description
The goal of this work was to develop a protocol for rapid genotyping of A and G variants at the CSN2 locus and genotyping of T and C variants at the CSN3 locus in sheep breeds (Sumava and Valachian) by means of PCR and LightCycler analysis. The LightCycler technique combines rapid and efficient in vitro amplification of DNA in glass capillaries with melting curve analysis based on fluorescence resonance energy transfer for the sensitive detection of point mutation. The A variant had a greater frequency (Sumava, 0.778; Valachian, 0.835) than did variant G (Sumava, 0.222; Valachian, 0.165) in both sheep breeds. The CSN3 locus was found to be monomorphic, with no polymorphism identified in either population. The goal of this work was to develop a protocol for rapid genotyping of A and G variants at the CSN2 locus and genotyping of T and C variants at the CSN3 locus in sheep breeds (Sumava and Valachian) by means of PCR and LightCycler analysis. The LightCycler technique combines rapid and efficient in vitro amplification of DNA in glass capillaries with melting curve analysis based on fluorescence resonance energy transfer for the sensitive detection of point mutation. The A variant had a greater frequency (Sumava, 0.778; Valachian, 0.835) than did variant G (Sumava, 0.222; Valachian, 0.165) in both sheep breeds. The CSN3 locus was found to be monomorphic, with no polymorphism identified in either population.
dcterms:title
Technical note: A novel method for routine genotyping of the G allele of β-casein (CSN2) and T allele of κ-casein (CSN3) in a sheep population using LightCycler Technical note: A novel method for routine genotyping of the G allele of β-casein (CSN2) and T allele of κ-casein (CSN3) in a sheep population using LightCycler
skos:prefLabel
Technical note: A novel method for routine genotyping of the G allele of β-casein (CSN2) and T allele of κ-casein (CSN3) in a sheep population using LightCycler Technical note: A novel method for routine genotyping of the G allele of β-casein (CSN2) and T allele of κ-casein (CSN3) in a sheep population using LightCycler
skos:notation
RIV/00027014:_____/11:#0001491!RIV12-MZE-00027014
n15:predkladatel
n16:ico%3A00027014
n4:aktivita
n6:Z
n4:aktivity
Z(MZE0002701404)
n4:cisloPeriodika
12
n4:dodaniDat
n5:2012
n4:domaciTvurceVysledku
n7:8284245 n7:9281851 n7:7749252 n7:2355000
n4:druhVysledku
n12:J
n4:duvernostUdaju
n20:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
234309
n4:idVysledku
RIV/00027014:_____/11:#0001491
n4:jazykVysledku
n13:eng
n4:klicovaSlova
beta-casein, κ-casein, genetic polymorphism, LightCycler
n4:klicoveSlovo
n9:LightCycler n9:beta-casein n9:%CE%BA-casein n9:genetic%20polymorphism
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[70CEECAAA65E]
n4:nazevZdroje
Journal of Animal Science
n4:obor
n8:EB
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
4
n4:rokUplatneniVysledku
n5:2011
n4:svazekPeriodika
89
n4:tvurceVysledku
Czerneková, Vladimíra Kyselová, Jitka Rychtářová, Jana Sztankóová, Zuzana
n4:wos
000297368200001
n4:zamer
n14:MZE0002701404
s:issn
0021-8812
s:numberOfPages
3
n18:doi
10.2527/jas.2011-4054