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Statements

Subject Item
n2:RIV%2F00027014%3A_____%2F11%3A%230001401%21RIV12-MZE-00027014
rdf:type
skos:Concept n15:Vysledek
rdfs:seeAlso
http://www.vuzv.cz/sites/File/_privat/11037.pdf
dcterms:description
Antlers as a potential model for bone growth and development have become an object of rising interest. To elucidate processes explaining how antler growth is regulated, in vitro cultures have been established. However, until now, there has been no standard method to cultivate antler cells and in vitro results are often opposite to those reported in vivo. In addition, many factors which are often not taken into account under in vitro conditions may play an important role in the development of antler cells. In this study we investigated the effects of the antler growth stage, the male individuality, passaged versus primary cultures and the effect of foetal calf serum concentrations on proliferative potential of mixed antler cell cultures in vitro, derived from regenerating antlers of red deer males (Cervus elaphus). The proliferation potential of antler cells was measured by incorporation of H-3 thymidine. Antlers as a potential model for bone growth and development have become an object of rising interest. To elucidate processes explaining how antler growth is regulated, in vitro cultures have been established. However, until now, there has been no standard method to cultivate antler cells and in vitro results are often opposite to those reported in vivo. In addition, many factors which are often not taken into account under in vitro conditions may play an important role in the development of antler cells. In this study we investigated the effects of the antler growth stage, the male individuality, passaged versus primary cultures and the effect of foetal calf serum concentrations on proliferative potential of mixed antler cell cultures in vitro, derived from regenerating antlers of red deer males (Cervus elaphus). The proliferation potential of antler cells was measured by incorporation of H-3 thymidine.
dcterms:title
Effect of Different Factors on Proliferation of Antler Cells, Cultured In Vitro Effect of Different Factors on Proliferation of Antler Cells, Cultured In Vitro
skos:prefLabel
Effect of Different Factors on Proliferation of Antler Cells, Cultured In Vitro Effect of Different Factors on Proliferation of Antler Cells, Cultured In Vitro
skos:notation
RIV/00027014:_____/11:#0001401!RIV12-MZE-00027014
n15:predkladatel
n16:ico%3A00027014
n4:aktivita
n9:Z n9:P
n4:aktivity
P(GA523/08/0808), Z(MZE0002701404)
n4:cisloPeriodika
3
n4:dodaniDat
n11:2012
n4:domaciTvurceVysledku
n14:5829879 n14:2372460 n14:5269849
n4:druhVysledku
n12:J
n4:duvernostUdaju
n8:S
n4:entitaPredkladatele
n20:predkladatel
n4:idSjednocenehoVysledku
196232
n4:idVysledku
RIV/00027014:_____/11:#0001401
n4:jazykVysledku
n18:eng
n4:klicovaSlova
regenerating deer antler, mesenchymal stem-cells, marrow stromal cells, fetal calf serum, bone-marrow, cervus-elaphus, velvet period,testosterone
n4:klicoveSlovo
n5:cervus-elaphus n5:fetal%20calf%20serum n5:regenerating%20deer%20antler n5:marrow%20stromal%20cells n5:mesenchymal%20stem-cells n5:bone-marrow n5:testosterone n5:velvet%20period
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[4ABABE385183]
n4:nazevZdroje
Plos One
n4:obor
n13:EG
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
4
n4:projekt
n19:GA523%2F08%2F0808
n4:rokUplatneniVysledku
n11:2011
n4:svazekPeriodika
6
n4:tvurceVysledku
Bubenik, G. A. Kotrba, Radim Bartoš, Luděk Kužmová, Erika
n4:wos
000289053800017
n4:zamer
n21:MZE0002701404
s:issn
1932-6203
s:numberOfPages
6
n10:doi
10.1371/journal.pone.0018053