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Statements

Subject Item
n2:RIV%2F00027014%3A_____%2F10%3A%230001178%21RIV11-GA0-00027014
rdf:type
skos:Concept n15:Vysledek
dcterms:description
The effect of different activation protocols on DNA integrity of porcine oocytes matured in vitro was analysed using the comet assay. The matured oocytes were then freed of cumulus cells and treated continuously or intermittently with a nitric oxide (NO) donor for 6 h. Standard activation with calcium ions (Ca2+) and culture without any treatment served as positive and negative controls, respectively. Exposure of oocytes to Ca2+ was associated with high activation efficiency, but decreased DNA integrity. The opposite was observed after continuous exposure to NO. Intermittent action of NO increased the activation rate, while the values of DNA damage remained at low levels. Our data suggest that an increased DNA instability could be the main reason compromising the further embryonic development of oocytes activated by the standard protocol. The intermittent treatment with NO thus represents a promising step to optimization of parthenogenetic activation of pig oocytes. The effect of different activation protocols on DNA integrity of porcine oocytes matured in vitro was analysed using the comet assay. The matured oocytes were then freed of cumulus cells and treated continuously or intermittently with a nitric oxide (NO) donor for 6 h. Standard activation with calcium ions (Ca2+) and culture without any treatment served as positive and negative controls, respectively. Exposure of oocytes to Ca2+ was associated with high activation efficiency, but decreased DNA integrity. The opposite was observed after continuous exposure to NO. Intermittent action of NO increased the activation rate, while the values of DNA damage remained at low levels. Our data suggest that an increased DNA instability could be the main reason compromising the further embryonic development of oocytes activated by the standard protocol. The intermittent treatment with NO thus represents a promising step to optimization of parthenogenetic activation of pig oocytes.
dcterms:title
Effect of different activation modes on DNA integrity of porcine M II oocytes matured in vitro Effect of different activation modes on DNA integrity of porcine M II oocytes matured in vitro
skos:prefLabel
Effect of different activation modes on DNA integrity of porcine M II oocytes matured in vitro Effect of different activation modes on DNA integrity of porcine M II oocytes matured in vitro
skos:notation
RIV/00027014:_____/10:#0001178!RIV11-GA0-00027014
n3:aktivita
n4:P n4:Z
n3:aktivity
P(GA523/06/0295), P(QI101A166), Z(AV0Z50390512), Z(MSM6046070901)
n3:cisloPeriodika
1
n3:dodaniDat
n10:2011
n3:domaciTvurceVysledku
n18:9755330
n3:druhVysledku
n16:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
255937
n3:idVysledku
RIV/00027014:_____/10:#0001178
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Comet assay, DNA integrity, Nitric oxide, Oocyte, Parthenogenesis, Pig
n3:klicoveSlovo
n5:DNA%20integrity n5:Parthenogenesis n5:Nitric%20oxide n5:Oocyte n5:Pig n5:Comet%20assay
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[25094E6C9BD3]
n3:nazevZdroje
Zygote
n3:obor
n13:ED
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n14:QI101A166 n14:GA523%2F06%2F0295
n3:rokUplatneniVysledku
n10:2010
n3:svazekPeriodika
18
n3:tvurceVysledku
Jílek, František Novotná, Božena Sedmíková, Markéta Kratochvílová, Jana Petr, Jaroslav
n3:wos
000274580200010
n3:zamer
n7:AV0Z50390512 n7:MSM6046070901
s:issn
0967-1994
s:numberOfPages
7