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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F14%3A00002970%21RIV15-MZE-00027006
rdf:type
skos:Concept n13:Vysledek
dcterms:description
Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5' extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic. Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5' extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic.
dcterms:title
Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines
skos:prefLabel
Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines
skos:notation
RIV/00027006:_____/14:00002970!RIV15-MZE-00027006
n3:aktivita
n15:Z
n3:aktivity
Z(MZE0002700604)
n3:cisloPeriodika
8
n3:dodaniDat
n11:2015
n3:domaciTvurceVysledku
n14:5557534
n3:druhVysledku
n18:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
30275
n3:idVysledku
RIV/00027006:_____/14:00002970
n3:jazykVysledku
n5:eng
n3:klicovaSlova
Trichovirus; Next-generation sequencing; Diversity; Detection
n3:klicoveSlovo
n7:Detection n7:Diversity n7:Trichovirus n7:Next-generation%20sequencing
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[4C52D7F5F8DF]
n3:nazevZdroje
Archives of Virology
n3:obor
n10:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n11:2014
n3:svazekPeriodika
159
n3:tvurceVysledku
Olmos, A. Predajňa, L. Komínek, Petr Nagyová, A. Candresse, T. Glasa, M.
n3:wos
000339888300024
n3:zamer
n6:MZE0002700604
s:issn
0304-8608
s:numberOfPages
5
n12:doi
10.1007/s00705-014-2031-5