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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F14%3A00002857%21RIV15-MZE-00027006
rdf:type
n9:Vysledek skos:Concept
dcterms:description
Glutathione S-transferases (GSTs) were purified using GSTrap 4B column, and the protein profile of the supernatant before purification was compared with the unbound fraction post-purification via two-dimensional gel electrophoresis (2D-E). The identity of these localized protein spots was confirmed by MS analysis and further analyzed by NanoLC-ESI-QUAD-TOF-MS/MS protein identification. Results indicate that 2 out of 5 protein spots were GSTs with region for class Delta and Epsilon subfamilies similar to other insects; however, the remaining 3 spots did not show any match in the current NCBInr. Both Delta and Epsilon class GSTs are specifically involved in insecticide resistance and their relatively high abundance in the 2D-E map suggests that these enzymes could play a role in the resistance of Meligethes aeneus to the most commonly used pyrethroids. The approach applied in this study for the specific localization of GSTs in 2D-E can be used for similar analyses in other organisms. Glutathione S-transferases (GSTs) were purified using GSTrap 4B column, and the protein profile of the supernatant before purification was compared with the unbound fraction post-purification via two-dimensional gel electrophoresis (2D-E). The identity of these localized protein spots was confirmed by MS analysis and further analyzed by NanoLC-ESI-QUAD-TOF-MS/MS protein identification. Results indicate that 2 out of 5 protein spots were GSTs with region for class Delta and Epsilon subfamilies similar to other insects; however, the remaining 3 spots did not show any match in the current NCBInr. Both Delta and Epsilon class GSTs are specifically involved in insecticide resistance and their relatively high abundance in the 2D-E map suggests that these enzymes could play a role in the resistance of Meligethes aeneus to the most commonly used pyrethroids. The approach applied in this study for the specific localization of GSTs in 2D-E can be used for similar analyses in other organisms.
dcterms:title
Methodology for glutathione S-transferase purification and localization in two-dimensional gel electrophoresis performed on the pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae) Methodology for glutathione S-transferase purification and localization in two-dimensional gel electrophoresis performed on the pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae)
skos:prefLabel
Methodology for glutathione S-transferase purification and localization in two-dimensional gel electrophoresis performed on the pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae) Methodology for glutathione S-transferase purification and localization in two-dimensional gel electrophoresis performed on the pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae)
skos:notation
RIV/00027006:_____/14:00002857!RIV15-MZE-00027006
n3:aktivita
n16:P
n3:aktivity
P(QH91152)
n3:cisloPeriodika
3
n3:dodaniDat
n5:2015
n3:domaciTvurceVysledku
n14:6991793 n14:6808565
n3:druhVysledku
n17:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
28648
n3:idVysledku
RIV/00027006:_____/14:00002857
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Glutathione S-transferase; Affinity chromatography; 2D-E; Meligethes aeneus; Proteomics; Delta and Epsilon GST subfamilies
n3:klicoveSlovo
n7:Delta%20and%20Epsilon%20GST%20subfamilies n7:Proteomics n7:Meligethes%20aeneus n7:Affinity%20chromatography n7:Glutathione%20S-transferase n7:2D-E
n3:kodStatuVydavatele
KR - Korejská republika
n3:kontrolniKodProRIV
[F7AFE87560B7]
n3:nazevZdroje
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
n3:obor
n15:GF
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n12:QH91152
n3:rokUplatneniVysledku
n5:2014
n3:svazekPeriodika
17
n3:tvurceVysledku
Erban, Tomáš Stará, Jitka
n3:wos
000340737700026
s:issn
1226-8615
s:numberOfPages
5
n8:doi
10.1016/j.aspen.2014.02.010