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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F12%3A00002159%21RIV13-MZE-00027006
rdf:type
n6:Vysledek skos:Concept
dcterms:description
A specific primer set, RetCO1F/RetCO/1R was designet by targeting consensus sequences from multiple aligments of the CO1 gene Lepinotus reticulatus for use in end point PCR, SYBR Green qPCR and HDA. Lepinotus a nd Liposcelis species were tested to confirm the primer specificity. The described primer set allowed accurate identification of L. reticulatus, producing an amplicon of 119 bp in the three described assays. A specific primer set, RetCO1F/RetCO/1R was designet by targeting consensus sequences from multiple aligments of the CO1 gene Lepinotus reticulatus for use in end point PCR, SYBR Green qPCR and HDA. Lepinotus a nd Liposcelis species were tested to confirm the primer specificity. The described primer set allowed accurate identification of L. reticulatus, producing an amplicon of 119 bp in the three described assays.
dcterms:title
PCR and isothermal-based molecular identification of the stored-product psocid pest Lepinotus reticulatus (Psocoptera: Trogiidae) PCR and isothermal-based molecular identification of the stored-product psocid pest Lepinotus reticulatus (Psocoptera: Trogiidae)
skos:prefLabel
PCR and isothermal-based molecular identification of the stored-product psocid pest Lepinotus reticulatus (Psocoptera: Trogiidae) PCR and isothermal-based molecular identification of the stored-product psocid pest Lepinotus reticulatus (Psocoptera: Trogiidae)
skos:notation
RIV/00027006:_____/12:00002159!RIV13-MZE-00027006
n6:predkladatel
n17:ico%3A00027006
n3:aktivita
n8:Z
n3:aktivity
Z(MZE0002700604)
n3:cisloPeriodika
April
n3:dodaniDat
n18:2013
n3:domaciTvurceVysledku
n14:2307596 n14:4568257
n3:druhVysledku
n13:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
158026
n3:idVysledku
RIV/00027006:_____/12:00002159
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Lepinotus; Molecular diagnostics; SYBR Green qPCR; CO1; Isothermal amplification
n3:klicoveSlovo
n5:CO1 n5:Molecular%20diagnostics n5:Isothermal%20amplification n5:Lepinotus n5:SYBR%20Green%20qPCR
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[FB2C6714D78E]
n3:nazevZdroje
Journal of Stored Products Research
n3:obor
n9:GF
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
7
n3:rokUplatneniVysledku
n18:2012
n3:svazekPeriodika
49
n3:tvurceVysledku
Stejskal, Václav Li, Z. Kučerová, Zuzana Ochoa-Corona, F. Opit, G. Yang, Q. Arif, M.
n3:wos
000304076600005
n3:zamer
n15:MZE0002700604
s:issn
0022-474X
s:numberOfPages
5
n7:doi
10.1016/j.jspr.2012.02.001