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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F12%3A00002114%21RIV13-MZE-00027006
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Dynamics of cold tolerance and crown proteome composition has been analysed in a set of two winter wheat cultivars Mironovskaya 808 and Bezostaya 1 and four reciprocal substitution lines with interchanged chromosomes 5A and 5B during a long-term cold-acclimation (CA) treatment. Proteome analysis has revealed 298 differently abundant spots during experiment. Most of them (260) were changed due to CA process and only 52 spots displayed differences between genotypes. Two hundred and seven protein spots were successfully identified by tandem mass spectrometry. Comparison of samples before and after vernalization fulfillment by a combination of ANOVA and Student’ T-test displayed ten differentially abundant protein spots (e.g. chopper chaperones). However, differences in the accumulation of these spots did not reflect differences in vernalization requirement of genotypes. Therefore, our results indicate that vernalization process has not influenced total proteome of CA wheat crowns. A few protein spots (14 spots; e.g. malate dehydrogenase) revealed differential accumulation levels between the individual genotypes or their groups possessing chromosome 5A or 5B from Mironovskaya 808 versus Bezostaya 1. The study has shown the effect of chromosome 5A on physiological traits and also proteome in winter wheat. Putative candidate protein markers for cold tolerance in wheat are discussed. Dynamics of cold tolerance and crown proteome composition has been analysed in a set of two winter wheat cultivars Mironovskaya 808 and Bezostaya 1 and four reciprocal substitution lines with interchanged chromosomes 5A and 5B during a long-term cold-acclimation (CA) treatment. Proteome analysis has revealed 298 differently abundant spots during experiment. Most of them (260) were changed due to CA process and only 52 spots displayed differences between genotypes. Two hundred and seven protein spots were successfully identified by tandem mass spectrometry. Comparison of samples before and after vernalization fulfillment by a combination of ANOVA and Student’ T-test displayed ten differentially abundant protein spots (e.g. chopper chaperones). However, differences in the accumulation of these spots did not reflect differences in vernalization requirement of genotypes. Therefore, our results indicate that vernalization process has not influenced total proteome of CA wheat crowns. A few protein spots (14 spots; e.g. malate dehydrogenase) revealed differential accumulation levels between the individual genotypes or their groups possessing chromosome 5A or 5B from Mironovskaya 808 versus Bezostaya 1. The study has shown the effect of chromosome 5A on physiological traits and also proteome in winter wheat. Putative candidate protein markers for cold tolerance in wheat are discussed.
dcterms:title
Analysis of proteome and frost tolerance in chromosome 5A and 5B reciprocal substitution lines between two winter wheats during long-term cold acclimation Analysis of proteome and frost tolerance in chromosome 5A and 5B reciprocal substitution lines between two winter wheats during long-term cold acclimation
skos:prefLabel
Analysis of proteome and frost tolerance in chromosome 5A and 5B reciprocal substitution lines between two winter wheats during long-term cold acclimation Analysis of proteome and frost tolerance in chromosome 5A and 5B reciprocal substitution lines between two winter wheats during long-term cold acclimation
skos:notation
RIV/00027006:_____/12:00002114!RIV13-MZE-00027006
n11:predkladatel
n15:ico%3A00027006
n3:aktivita
n19:P n19:Z
n3:aktivity
P(GA522/08/1290), P(GP522/09/P621), P(OC08066), Z(MZE0002700604)
n3:cisloPeriodika
1
n3:dodaniDat
n10:2013
n3:domaciTvurceVysledku
n12:5347084 n12:9107169 n12:5003717
n3:druhVysledku
n17:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
122202
n3:idVysledku
RIV/00027006:_____/12:00002114
n3:jazykVysledku
n16:eng
n3:klicovaSlova
Cold-acclimation; Crowns; Plant proteomics; Plant stress; Two-dimensional differential in-gel electrophoresis; Winter wheat
n3:klicoveSlovo
n8:Crowns n8:Cold-acclimation n8:Two-dimensional%20differential%20in-gel%20electrophoresis n8:Winter%20wheat n8:Plant%20proteomics n8:Plant%20stress
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[216FF2FEF641]
n3:nazevZdroje
Proteomics
n3:obor
n18:ED
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
5
n3:projekt
n7:GA522%2F08%2F1290 n7:OC08066 n7:GP522%2F09%2FP621
n3:rokUplatneniVysledku
n10:2012
n3:svazekPeriodika
12
n3:tvurceVysledku
Renaut, J. Vítámvás, Pavel Planchon, S. Prášil, Ilja Kosová, Klára
n3:wos
000298841000011
n3:zamer
n13:MZE0002700604
s:issn
1615-9853
s:numberOfPages
18
n20:doi
10.1002/pmic.201000779