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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F10%3A00001324%21RIV11-MZE-00027006
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Stored-food and house-dust arthropods include many species of mites and beetles that affect human health. For diagnostic tests proteases such as trypsin are utilized as they are indicators of the presence of allergen contaminants in food. We recently characterized Kunitz-type protease inhibitors (KPIs) from Solanum palustre. Here we studied biotechnological applications of KPI-B1 and -B4. We manufactured a protein chip with immobilized KPI-B1 and -B4 and showed trypsin/chymotrypsin-binding specificity, indicating that the recombinant proteins have protease selectivity. We employed the protein chip to capture mite proteins belonging to the protease family with polyclonal anti-mite antibodies. The mite diagnostic chip can be useful for detecting mite allergens. Stored-food and house-dust arthropods include many species of mites and beetles that affect human health. For diagnostic tests proteases such as trypsin are utilized as they are indicators of the presence of allergen contaminants in food. We recently characterized Kunitz-type protease inhibitors (KPIs) from Solanum palustre. Here we studied biotechnological applications of KPI-B1 and -B4. We manufactured a protein chip with immobilized KPI-B1 and -B4 and showed trypsin/chymotrypsin-binding specificity, indicating that the recombinant proteins have protease selectivity. We employed the protein chip to capture mite proteins belonging to the protease family with polyclonal anti-mite antibodies. The mite diagnostic chip can be useful for detecting mite allergens.
dcterms:title
Protein chips for detection of mite allergens using Kunitz-type protease inhibitors Protein chips for detection of mite allergens using Kunitz-type protease inhibitors
skos:prefLabel
Protein chips for detection of mite allergens using Kunitz-type protease inhibitors Protein chips for detection of mite allergens using Kunitz-type protease inhibitors
skos:notation
RIV/00027006:_____/10:00001324!RIV11-MZE-00027006
n3:aktivita
n11:Z
n3:aktivity
Z(MZE0002700604)
n3:cisloPeriodika
6
n3:dodaniDat
n7:2011
n3:domaciTvurceVysledku
n15:7995520
n3:druhVysledku
n8:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
283059
n3:idVysledku
RIV/00027006:_____/10:00001324
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Analytical biotechnology; biosensors; protease; protease inhibitor; protein chips
n3:klicoveSlovo
n4:protease n4:protease%20inhibitor n4:protein%20chips n4:Analytical%20biotechnology n4:biosensors
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[56837B284032]
n3:nazevZdroje
Biotechnology Journal
n3:obor
n14:GF
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
8
n3:rokUplatneniVysledku
n7:2010
n3:svazekPeriodika
5
n3:tvurceVysledku
Liu, S. Santino, A. Křížková Kudlíková, Iva Wang, Y. De Blasi, M. Poltronieri, P. Cimaglia, F.
n3:zamer
n10:MZE0002700604
s:issn
1860-6768
s:numberOfPages
6