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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F09%3A00000709%21RIV10-MZE-00027006
rdf:type
n7:Vysledek skos:Concept
dcterms:description
The LN33 grapevine plant was artificially infected with Grapevine leafroll-associated virus-1 (GLRaV-1), Grapevine virus A (GVA), Grapevine virus B (GVB), Rupestris stem pitting-associated virus (RSPaV), and an unclassified tymovirus. Although infected with GVB, corky bark symptoms never appeared, and this virus had no destructive effects on the LN33 grapevines under our experimental conditions. Dormant canes, sampled before the beginning of the vegetation period, were used as suitable targets to detect mentioned viruses using both the ELISA and RT-PCR method. For GLRaV-1, the ELISA- based diagnosis unexpectedly appeared more effective than RT-PCR; probably due to the insufficient specificity of the primers used, not reflecting the actual genetic variability of the virus. Distribution of viruses in long-term infected grapevine plants showed different degrees of irregularity, depending on the individual viruses. The LN33 grapevine plant was artificially infected with Grapevine leafroll-associated virus-1 (GLRaV-1), Grapevine virus A (GVA), Grapevine virus B (GVB), Rupestris stem pitting-associated virus (RSPaV), and an unclassified tymovirus. Although infected with GVB, corky bark symptoms never appeared, and this virus had no destructive effects on the LN33 grapevines under our experimental conditions. Dormant canes, sampled before the beginning of the vegetation period, were used as suitable targets to detect mentioned viruses using both the ELISA and RT-PCR method. For GLRaV-1, the ELISA- based diagnosis unexpectedly appeared more effective than RT-PCR; probably due to the insufficient specificity of the primers used, not reflecting the actual genetic variability of the virus. Distribution of viruses in long-term infected grapevine plants showed different degrees of irregularity, depending on the individual viruses.
dcterms:title
Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution
skos:prefLabel
Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution
skos:notation
RIV/00027006:_____/09:00000709!RIV10-MZE-00027006
n3:aktivita
n16:Z n16:P
n3:aktivity
P(QG50083), Z(MZE0002700604)
n3:cisloPeriodika
4
n3:dodaniDat
n12:2010
n3:domaciTvurceVysledku
n11:2856042 n11:5557534
n3:druhVysledku
n6:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
302902
n3:idVysledku
RIV/00027006:_____/09:00000709
n3:jazykVysledku
n4:eng
n3:klicovaSlova
grapevine; virus; corky bark; ELISA; RT-PCR; detection
n3:klicoveSlovo
n8:grapevine n8:corky%20bark n8:detection n8:RT-PCR n8:virus n8:ELISA
n3:kodStatuVydavatele
SK - Slovenská republika
n3:kontrolniKodProRIV
[072B8B1C37FC]
n3:nazevZdroje
Acta Virologica
n3:obor
n13:GF
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n17:QG50083
n3:rokUplatneniVysledku
n12:2009
n3:svazekPeriodika
53
n3:tvurceVysledku
Glasa, M. Komínek, Petr Komínková, Marcela
n3:wos
000272972600010
n3:zamer
n14:MZE0002700604
s:issn
0001-723X
s:numberOfPages
5