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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F08%3A00000319%21RIV09-MZE-00027006
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Byly připraveny dvě polyklonální protilátky pro detekci Tribolium castaneum. Technika PTA-ELISA byla použita pro detekci dospělců, larev a exkrementů T. castaneum v uměle kontaminovaných vzorcích obilí. Pro každý typ kontaminace byla provedena kalibrace a určení detekčního limitu (60 jedinců pro larvy, 640 jedinců pro dospělce, 4mg pro exkrementy/ kg obilí). Což po přepočtu znamená, že technika je schopna odhalit kontaminaci způsobenou 30 larvami žijícími po 5 dnů v optimálních podmínkách. Hlavní předností vyvinuté techniky je schopnost stopování kontaminace způsobené T. castaneum i v případě, že jedinci byly ze substrátu odebráni. Two polyclonal antibodies (Pab) were developed for the detection of Tribolium castaneum, which is a stored product pest of medical and economical importance. PTA-ELISA was used to detect adults, larva, and feces of T. castaneum in artificially contaminated grain samples. Calibration methods were applied to determine detection limits for each type of contaminants. Anti-T.castaneumK51 enabled detection of T. castaneum in grain samples: detection limits reached 60 and 640 individuals/kg of grain for larvae and adults, respectively, and 4 mg of feces/kg of grain. After recalculation, the detection limit for feces enables detection of 30 larvae after 5 days of feeding in optimal conditions. The main advantage of the developed assay is traceability of T. castaneum contamination, especially when the adults and larvae are removed from grain. Two polyclonal antibodies (Pab) were developed for the detection of Tribolium castaneum, which is a stored product pest of medical and economical importance. PTA-ELISA was used to detect adults, larva, and feces of T. castaneum in artificially contaminated grain samples. Calibration methods were applied to determine detection limits for each type of contaminants. Anti-T.castaneumK51 enabled detection of T. castaneum in grain samples: detection limits reached 60 and 640 individuals/kg of grain for larvae and adults, respectively, and 4 mg of feces/kg of grain. After recalculation, the detection limit for feces enables detection of 30 larvae after 5 days of feeding in optimal conditions. The main advantage of the developed assay is traceability of T. castaneum contamination, especially when the adults and larvae are removed from grain.
dcterms:title
Development of polyclonal antibodies for the detection of Tribolium castaneum contamination in wheat grain Vývoj protilátek pro detekci kontaminace způsobené Tribolium castaneum v obilí Development of polyclonal antibodies for the detection of Tribolium castaneum contamination in wheat grain
skos:prefLabel
Vývoj protilátek pro detekci kontaminace způsobené Tribolium castaneum v obilí Development of polyclonal antibodies for the detection of Tribolium castaneum contamination in wheat grain Development of polyclonal antibodies for the detection of Tribolium castaneum contamination in wheat grain
skos:notation
RIV/00027006:_____/08:00000319!RIV09-MZE-00027006
n3:aktivita
n15:P
n3:aktivity
P(1B53040)
n3:cisloPeriodika
léto
n3:dodaniDat
n11:2009
n3:domaciTvurceVysledku
n6:7995520 n6:4892747
n3:druhVysledku
n13:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
363160
n3:idVysledku
RIV/00027006:_____/08:00000319
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Detection; grain; Tribolium castaneum; storage; pest; ELISA
n3:klicoveSlovo
n9:Tribolium%20castaneum n9:Detection n9:pest n9:storage n9:ELISA n9:grain
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[4D87B2442832]
n3:nazevZdroje
Journal of Agricultural and Food Chemistry
n3:obor
n5:GF
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n16:1B53040
n3:rokUplatneniVysledku
n11:2008
n3:svazekPeriodika
56
n3:tvurceVysledku
Křížková Kudlíková, Iva Hubert, Jan
s:issn
0021-8561
s:numberOfPages
6