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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F08%3A00000268%21RIV09-MZE-00027006
rdf:type
skos:Concept n15:Vysledek
dcterms:description
Erwinia amylovora (Ea) causing fire blight and Clavibacter michiganensis subsp. sepedoniciis (Cms) causing ring rot of potato belong to the quarantine organisms. Detection and determination of both pathogens using different methods were compared. Commercial MAb were used for Cms and PAb for Ea. All Ea strains were reliably identified by PCR (Bereswill and own primers) after optimisation of PCR protocol and using BIOLOG System. PTA-ELISA and IIF were less reliable because of cross-reacting bacteria associated with Ea in host plants. AH Cms strains were reliably identified by IIF and PCR (Mills and Pastrik primers) regardless of morphological variability. The fluidal Cms strains were more reliably identified using DAS-ELISA and BIOLOG System than less fluidal strains. Erwinia amylovora (Ea), původce spály růžovitých rostlin a Clavibacter michiganensis subsp. sepedoniciis (Cms), původce kroužkovitosti bramboru, patří ke karanténním organismům. Porovnávala se detekce a determinace obou patogenů pomocí různých diagnostických metod. Komerční MAb byly použity pro Cms a PAb pro Ea. Všechny Ea kmeny byly spolehlivě identifikovány pomocí PCR po optimalizaci PCR protokolu a systémem BIOLOG. PTA-ELISA a IIF byly méně spolejlivé kvůli křížových reakcím bakterií doprovázejících Ea v hostitelských rostlinách. Všechny Cms kmeny byly spolehlivě identifikovány pomocí IIF a PCR bez ohledu na morfologickou variabilitu. Fluidní kmeny Cms byly spolehlivěji identifikovány pomocí DAS-ELISA a systému BIOLOG než nefluidní. Erwinia amylovora (Ea) causing fire blight and Clavibacter michiganensis subsp. sepedoniciis (Cms) causing ring rot of potato belong to the quarantine organisms. Detection and determination of both pathogens using different methods were compared. Commercial MAb were used for Cms and PAb for Ea. All Ea strains were reliably identified by PCR (Bereswill and own primers) after optimisation of PCR protocol and using BIOLOG System. PTA-ELISA and IIF were less reliable because of cross-reacting bacteria associated with Ea in host plants. AH Cms strains were reliably identified by IIF and PCR (Mills and Pastrik primers) regardless of morphological variability. The fluidal Cms strains were more reliably identified using DAS-ELISA and BIOLOG System than less fluidal strains.
dcterms:title
Spolehlivost diagnostických technik pro Erwinia amylovora a Clavibacter michiganensis subsp. sepedonicus Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus
skos:prefLabel
Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus Spolehlivost diagnostických technik pro Erwinia amylovora a Clavibacter michiganensis subsp. sepedonicus
skos:notation
RIV/00027006:_____/08:00000268!RIV09-MZE-00027006
n3:aktivita
n16:Z
n3:aktivity
Z(MZE0002700603)
n3:cisloPeriodika
3
n3:dodaniDat
n9:2009
n3:domaciTvurceVysledku
n6:1201948
n3:druhVysledku
n4:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
392251
n3:idVysledku
RIV/00027006:_____/08:00000268
n3:jazykVysledku
n10:eng
n3:klicovaSlova
Erwinia amylovora; Clavibacter michiganensis subsp. sepedonicus; detection; determination; PCR; BIOLOG; ELISA; IF
n3:klicoveSlovo
n5:Clavibacter%20michiganensis%20subsp.%20sepedonicus n5:ELISA n5:BIOLOG n5:detection n5:PCR n5:IF n5:determination n5:Erwinia%20amylovora
n3:kodStatuVydavatele
LT - Litevská republika
n3:kontrolniKodProRIV
[4C6DFE894AEB]
n3:nazevZdroje
Zemdirbyste-Agriculture
n3:obor
n11:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
2
n3:rokUplatneniVysledku
n9:2008
n3:svazekPeriodika
95
n3:tvurceVysledku
Mráz, I. Kokošková, Blanka
n3:wos
000259827600038
n3:zamer
n12:MZE0002700603
s:issn
1392-3196
s:numberOfPages
7