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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F07%3A2420%21RIV08-MZE-00027006
rdf:type
n14:Vysledek skos:Concept
dcterms:description
Práce se zabývá porovnáním detekčních metod pro identifikaci kontaminace roztočem Acarus siro. Jsou testovány klasické detekční metody (extrakce na Berlese-Tullgren extraktoru, prosévání a flotace). Další použitou metodou byla ELISA s nově připravenou polyklonální protilátkou pro detekci A. siro. Bylo zjištěno, že dospělce a juvenilní stádia bylo možné detekovat všemi uvedenými metodami s rozdílným detekčním limitem v rozsahu 221 až 1157 roztočů/kg obilí. Vajíčka bylo možné detekovat pouze pomocí flotace (detekční limit 1950 vajíček/kg obilí). Exkrementy roztoče byly detekovatelné pouze pomocí imunochemického testu s detekčním limitem 1.04 µg exkrementů/g obilí, což znamená, že použitá technika umožní detekci jednoho metabolicky aktivního roztoče/g obilí. Acarus siro L. (Acari: Acaridida: Acarididae) is an important pest of stored grain because it contaminates the grain by allergens and transfers pathogenous microorganisms. Rapid detection of contamination enables to intercept an early grain infestation by the pest. In this study, we compared the usability and efciency of various detection approaches. Under laboratory conditions, grain samples of various sizes were infested by different levels of the following contaminants: eggs, adults, and feces of A. siro. The samples were analyzed by enzyme-linked immunosorbent essay (ELISA) by using anti-A. siro polyclonal antibody (immunochemical method), extracted in Berlese-Tullgren funnels, sieved, and processed by the flotation (conventional methods). The adults or juveniles of A. siro could be detected by all the three tested conventional methods and ELISA with detection limits in the range from 221 to 1,157 mites/kg grain. Eggs were detected by the flotation only; the detection limit was 1,950 eggs/kg gra Acarus siro L. (Acari: Acaridida: Acarididae) is an important pest of stored grain because it contaminates the grain by allergens and transfers pathogenous microorganisms. Rapid detection of contamination enables to intercept an early grain infestation by the pest. In this study, we compared the usability and efciency of various detection approaches. Under laboratory conditions, grain samples of various sizes were infested by different levels of the following contaminants: eggs, adults, and feces of A. siro. The samples were analyzed by enzyme-linked immunosorbent essay (ELISA) by using anti-A. siro polyclonal antibody (immunochemical method), extracted in Berlese-Tullgren funnels, sieved, and processed by the flotation (conventional methods). The adults or juveniles of A. siro could be detected by all the three tested conventional methods and ELISA with detection limits in the range from 221 to 1,157 mites/kg grain. Eggs were detected by the flotation only; the detection limit was 1,950 eggs/kg gra
dcterms:title
Comparison of Detection Methods for Acarus siro (Acari:Acaridida: Acarididae) Contamination in Grain Comparison of Detection Methods for Acarus siro (Acari:Acaridida: Acarididae) Contamination in Grain Porovnání detekčních metod pro roztoče Acarus siro (Acari: Acaridida: Acarididae) v obilí
skos:prefLabel
Comparison of Detection Methods for Acarus siro (Acari:Acaridida: Acarididae) Contamination in Grain Comparison of Detection Methods for Acarus siro (Acari:Acaridida: Acarididae) Contamination in Grain Porovnání detekčních metod pro roztoče Acarus siro (Acari: Acaridida: Acarididae) v obilí
skos:notation
RIV/00027006:_____/07:2420!RIV08-MZE-00027006
n3:strany
1928;1937
n3:aktivita
n11:P
n3:aktivity
P(QF4071)
n3:cisloPeriodika
6
n3:dodaniDat
n16:2008
n3:domaciTvurceVysledku
n12:7995520 n12:2307596 n12:4892747
n3:druhVysledku
n7:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
414310
n3:idVysledku
RIV/00027006:_____/07:2420
n3:jazykVysledku
n8:eng
n3:klicovaSlova
extraction; ELISA; grain; mite; storage
n3:klicoveSlovo
n5:extraction n5:storage n5:ELISA n5:mite n5:grain
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[D558CEE4C435]
n3:nazevZdroje
Journal of Economic Entomology
n3:obor
n10:GF
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n15:QF4071
n3:rokUplatneniVysledku
n16:2007
n3:svazekPeriodika
100
n3:tvurceVysledku
Křížková Kudlíková, Iva Stejskal, Václav Hubert, Jan
s:issn
0022-0493
s:numberOfPages
10