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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F06%3A1736%21RIV07-MZE-00027006
rdf:type
skos:Concept n12:Vysledek
dcterms:description
Shoot tips of apple cultivar 'Rubin' were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg L-1 BAP and 0.1 mg L-1 IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg L-1 BAP, which produced 4.2 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. 'Rubin' were cultivated for 4 weeks at low temperature (4°C) and short photoperiod (8/16-h – light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. In vitro plants were exposed to the controlled cooling rate 4°C/h. The lethal temperature (LT) was -13.0°C for cold hardened and 6.5°C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of c Shoot tips of apple cultivar 'Rubin' were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg L-1 BAP and 0.1 mg L-1 IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg L-1 BAP, which produced 4.2 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. 'Rubin' were cultivated for 4 weeks at low temperature (4°C) and short photoperiod (8/16-h – light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. In vitro plants were exposed to the controlled cooling rate 4°C/h. The lethal temperature (LT) was -13.0°C for cold hardened and 6.5°C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of c Shoot tips of apple cultivar 'Rubin' were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg L-1 BAP and 0.1 mg L-1 IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg L-1 BAP, which produced 4.2 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. 'Rubin' were cultivated for 4 weeks at low temperature (4°C) and short photoperiod (8/16-h – light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. In vitro plants were exposed to the controlled cooling rate 4°C/h. The lethal temperature (LT) was -13.0°C for cold hardened and 6.5°C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of c
dcterms:title
Proliferation and cold hardening of in vitro grown apple shoot tips Proliferace a mrazové otužování in vitro kultur jabloní Proliferation and cold hardening of in vitro grown apple shoot tips
skos:prefLabel
Proliferation and cold hardening of in vitro grown apple shoot tips Proliferation and cold hardening of in vitro grown apple shoot tips Proliferace a mrazové otužování in vitro kultur jabloní
skos:notation
RIV/00027006:_____/06:1736!RIV07-MZE-00027006
n3:strany
0
n3:aktivita
n17:P
n3:aktivity
P(QC1052)
n3:cisloPeriodika
0
n3:dodaniDat
n13:2007
n3:domaciTvurceVysledku
n8:5390079 n8:7227663
n3:druhVysledku
n5:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
495489
n3:idVysledku
RIV/00027006:_____/06:1736
n3:jazykVysledku
n9:eng
n3:klicovaSlova
micropropagation, germplasm, encapsulation, cryopreservation, freezing
n3:klicoveSlovo
n4:freezing n4:encapsulation n4:micropropagation n4:germplasm n4:cryopreservation
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[EEEB6334C38F]
n3:nazevZdroje
Acta Horticulturae
n3:obor
n7:ED
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n16:QC1052
n3:rokUplatneniVysledku
n13:2006
n3:tvurceVysledku
Bilavčík, Alois Sedlák, Jiří Zámečník, Jiří Paprštein, František
s:issn
0567-7572
s:numberOfPages
4